Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli
Journal of BacteriologyPublished 1 October 1980Open access
Klaus B. Heller, E. C. C. Lin, T. Hastings Wilson
Citations332
SJR quartileQ2
SJR score1.09
SNIP0.85
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TL;DR
The observation and the insensitivity of the xylitol transport to low temperature suggest that the facilitator behaves as a membrane channel.
Abstract
The specificity of the glycerol facilitator (glpF) of Escherichia coli was studied with an osmotic method. This transport system allowed the entry of polyols (glycerol and erythritol), pentitols, and hexitols. The analogous sugars were not transported. However, urea, glycine, and DL-glyceraldehyde could use this pathway to enter the cell. The glpF protein allowed the rapid efflux of preequilibrated xylitol. Glycerol surprisingly did not inhibit the uptake of xylitol, and xylitol only slightly reduced the uptake of glycerol. The observation and the insensitivity of the xylitol transport to low temperature suggest that the facilitator behaves as a membrane channel.
Keywords
Biochemistry, Genetics and Molecular Biology
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