Isopentenyladenine from mutants of the moss, Physcomitrella patens
PhytochemistryPublished 1 January 1980
Trevor L. Wang, David J. Cove, Peter Beutelmann, Elmar Hartmann
Citations51
SJR quartileQ1
SJR score0.73
SNIP1.23
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TL;DR
The culture media from gametophore over-producing mutants of the moss Physcomitrella patens have been examined for their cytokinin content and two cytokinins have been detected, one of which has been identified as N6-(Δ2-isopentenyl) adenine (2iP).
Abstract
The culture media from gametophore over-producing mutants of the moss Physcomitrella patens have been examined for their cytokinin content. Two cytokinins have been detected, one of which has been identified as N6-(Δ2-isopentenyl) adenine (2iP).
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
Molecular and General Genetics MGGThe isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss, Physcomitrella patens
445 Citations1977Neil W. Ashton, David J. Cove
A diploid strain has been obtained by aposporus regeneration from a hybrid sporophyte and the phenotypes of progeny resulting from the self-fertilisation of this strain have been analysed.
PlantaAnalysis of gametophytic development in the moss, Physcomitrella patens, using auxin and cytokinin resistant mutants
326 Citations1979Neil W. Ashton, Nigel Grimsley +1 more
Physiological analysis of mutants isolated in the moss Physcomitrella patens has shown that both endogenous auxin and cytokinin are likely to play important and interdependent roles in several steps of gametophytic development.
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141 Citations1977Nigel Grimsley, Neil W. Ashton +1 more
Somatic hybrids selected following treatment of mixtures of protoplasts from complementary auxotrophic strains with 50 mM CaCl2 at high pH have a morphology different from that of normal haploid strains, but similar to that of aposporously produced diploids.
PLANT PHYSIOLOGYStudies on Cytokinin-Controlled Bud Formation in Moss Protonemata
119 Citations1968Hans Brandes, Hans Kende
Autoradiographs of protonemata treated with a labeled cytokinin, benzyladenine-benzyl-7-(14)C, show a striking accumulation of the radioactivity in caulonema cells which are in the stage of bud formation, and in the buds themselves.
PlantaThe isolation and physiological analysis of mutants of the moss, Physcomitrella patens, which over-produce gametophores
97 Citations1979Neil W. Ashton, David J. Cove +1 more
Several phenotypically distinct classes of gametophore overproducing mutants have been isolated in P. patens and it is shown that these mutants are unable to synthesize/activate this component in the dark and thus, like the wild-type strain, they produce nogametophores in thedark.
PlantaA cytokinin test with high specificity
88 Citations1968Heinz Hahn, Martin Bopp
A cytokinin bioassay based on bud formation in 10-cell-long caulonema filaments of Funaria hygrometrica is described, which has high specificity and sensitivity and exhibits linearity between cytokinIn concentration in the medium and bud number.
Botanical GazetteDevelopment of the Gametophyte in the Moss Tortella caespitosa
78 Citations1957Bert S. Gorton, Robert E. Eakin
It was shown that adenine and various related compounds can stimulate bud formation and kinetin is especially effective in promoting cell division in buds but not in the protonemata; a growth-inhibitory material is built up in the medium during the growth of the moss.
PlantaPurification and identification of a cytokinin from moss callus cells
48 Citations1977Peter Beutelmann, L. Bauer
A cytokinin was isolated from the culture medium of callus cells of the moss hybrid Funaria hygrometrica and confirmed as N6−(Δ2-isopentenyl)adenine by means of gas chromatography and mass spectrometry.
PlantaIn vitro cytokinin binding to a particulate cell fraction from protonemata of Funaria hygrometrica
23 Citations1978Gary Gardner, Michael R. Sussman +1 more
The problem remains, in all studies of cytokinin binding, to distinguish between binding that is biologically meaningful, and biological (biologically) non-meaningful physical adsorption.
Physiologia PlantarumConnection between the Synthesis of Differentiation Specific Proteins and the Capacity of Cells to Respond to Cytokinin in the Moss <i>Funaria</i>
23 Citations1978Martin Bopp, Uta Erichsen +2 more
The differentiation stage of the caulonema in Funaria hygrometrica protonema is distinguished from the chloronema stage by three additional protein bands (CSP) in the soluble protein fraction, and it is concluded that the CSP are involved in the competence of caul onema cells to respond to cytokinins.
Plant Science LettersEvidence for cytokinins in gametophytes of the fern Anemia phyllitidis L. Sw.
6 Citations1979H. Schraudolf, Angela-Melanie Fischer
Gametophytes of Anemia phyllitidis contain free cytokinins and zeatin which is accompanied by its riboside and 2-isopentenyladenosine could be identified by TLC as the main active compounds.