Enhanced phosphatidylinositol labelling in rat parotid fragments exposed to α-adrenergic stimulation
Biochemical JournalPublished 1 January 1974Open access
Robert H. Michell, Lynne Jones
Citations51
SJR quartileQ1
SJR score2.06
SNIP0.98
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
It is suggested that the stimulation of phospholipid metabolism that occurs in several other tissues in the presence of adrenaline or noradrenaline may also involve alpha-receptors.
Abstract
1. Adrenergic agonists provoke a marked increase in labelling of phosphatidylinositol in fragments of rat parotid gland. 2. Adrenaline and phenylephrine (an adrenergic alpha-agonist) are effective stimulants, but isoprenaline (an adrenergic beta-agonist) is relatively ineffective. 3. The response evoked by phenylephrine or adrenaline is prevented by prior incubation of the tissue with phenoxybenzamine (an alpha-receptor blocking agent), but not by prior incubation with pindolol (a beta-receptor blocking agent). 4. Adrenergic stimulation of phosphatidylinositol metabolism in parotid gland is therefore mediated through alpha-receptors, in common with the adrenaline-induced K(+) efflux. It is not linked to enzyme secretion, which is triggered by stimulation of beta-receptors. 5. It is suggested that the stimulation of phospholipid metabolism that occurs in several other tissues in the presence of adrenaline or noradrenaline may also involve alpha-receptors.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
European Journal of BiochemistryThe Mechanism of Enzyme Secretion by the Cell
187 Citations1967Helene Babad, Ruth Ben-Zvi +2 more
Experiments indicated that 3′5′ cyclic AMP is an intermediate in the induction of enzyme secretion by epinephrine, and both epine cortisol and norepinephrine were more effective than other agents tested.
Journal of Biological ChemistryPotassium Release Mediated by the Epinephrine α-Receptor in Rat Parotid Slices
161 Citations1973Shmuel Batzri, Zvi Selinger +2 more
Electron microscopy showed that phenylephrine which activates the α-adrenergic receptor causes extensive vacuolation of the acinar gland cells and isoproterenol and N6-monobutyryl cAMP cause fusion of the secretory granules with the cell membrane facing the lumen but no vacuolated.
Biochemical JournalA membrane-bound activity catalysing phosphatidylinositol breakdown to 1,2-diacylglycerol, <scp>d</scp>-<i>myo</i>inositol 1:2-cyclic phosphate and <scp>d</scp>-<i>myo</i>inositol 1-phosphate. Properties and subcellular distribution in rat cerebral cortex
142 Citations1973Eduardo G. Lapetina, Robert H. Michell
International review of cytologyDynamic Aspects of Phospholipids during Protein Secretion
138 Citations1968Lowell E. Hokin
This chapter focuses on the dynamic aspects of phospholipids in exocrine cells which secrete organic molecules, in particular the exocrine pancreas, which secretes protein.
Journal of Biological ChemistryEnzyme Secretion Mediated by the Epinephrine β-Receptor in Rat Parotid Slices
133 Citations1973Shmuel Batzri, Zvi Selinger
It is tentatively concluded that the Na+,K+-activated ATPase is not involved in enzyme secretion and is shown in the accompanying papers that the potassium release is specifically induced by an epinephrine cu-receptor.
Biochemical JournalThe diglyceride kinase of rat cerebral cortex
115 Citations1971E G Lapetina, J. N. Hawthorne
The rate of phosphatidic acid synthesis by the diglyceride kinase route was much greater than reported rates for acylation of 3-glycerophosphate and was also very rapid in comparison with the rates of other steps in the synthesis of phosphoinositides.
Journal of Biological ChemistryStimulation of 32Pi Incorporation into Phosphatidylinositol and Phosphatidylglycerol by Catecholamines and β-Adrenergic Receptor Blocking Agents in Rat Pineal Organ Cultures
89 Citations1973Joseph Eichberg, Harvey M. Shein +2 more
The findings indicate that the increase in phospholipid radioactivity is not related to the formation of melatonin which in the cultured pineal is stimulated by either norepinephrine or N6, O2'-dibutyryl adenosine 3',5'-monophosphate, and this conclusion is supported by the fact that propranolol fails to block enhanced phospholIPid labeling whereas it blocks enhanced melatonin formation.
Biochemical JournalStimulation by acetylcholine of phosphatidylinositol labelling. Subcellular distribution in rat cerebral-cortex slices
80 Citations1972Eduardo G. Lapetina, Robert H. Michell
The hypothesis that the labelled phosphatidylinositol produced by stimulation is a component of an acetylcholine-receptor proteolipid localized in the synaptic junction is supported.
The Journal of PhysiologyProtein secretion and phosphate turnover in the phospholipids in salivary glands <i>in vitro</i>
80 Citations1957L E Hokin, Allan L. Sherwin
Observations on protein secretion and the incorporation of 32p into the phospholipids of slices of salivary glands give further support to the hypothesis (Hokin & Hokin, 1956a, b) that phosphate turnover in the phosphlipids plays a functional role in protein secretion.
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid MetabolismExtraction of polyphosphoinositides with neutral and acidified solvents A comparison of guinea-pig brain and liver, and measurements of rat liver inositol compounds which are resistant to extraction
76 Citations1970Robert H. Michell, J. N. Hawthorne +2 more
Analysis of the residue remaining after extraction of lipids from rat liver and of phosphate ester fractions obtained from this tissue by acid hydrolysis showed only low concentrations of inositol attributable to inositols di- and triphosphates.
Journal of NeurochemistryEFFECT OF NOREPINEPHRINE ON <sup>32</sup>P INCORPORATION INTO INDIVIDUAL PHOSPHATIDES IN SLICES FROM DIFFERENT AREAS OF THE GUINEA PIG BRAIN<sup>1</sup>
73 Citations1969Mabel R. Hokin
Norepinephrine (1 mm) significantly inhibited 32P incorporation into phosphatidylcholine in cerebral cortex, hypothalamus, corpus striatum and anterior colliculus; 32Porporation intoosphatidylethanolamine plus phosphatide metabolism in response to neurotransmitters in the central nervous system was inhibited by 1 mm‐norpinephrine.
Nature New BiologyProduction of Cyclic Inositol Phosphate in Stimulated Tissues
69 Citations1972Robert H. Michell, Eduardo G. Lapetina
The increased phosphatidylinositol turnover which occurs in many stimulated tissues may be a reflexion of the intracellular production of myoinositol-1,2-cyclic phosphate.
Biochimica et Biophysica Acta (BBA) - General SubjectsRole of cyclic 3′,5t-AMP in the action of physiological secretagogues on the metabolism of rat pancreas in vitro☆
64 Citations1971H Bauduin, L. Rochus +2 more
The involvement of cyclic AMP as mediator of cholinergic agents and pancreozymin, the relationship between stimulation of secretion and the rate of protein synthesis and the relationship of the phospholipid effect with secretion are discussed.
Archives of Biochemistry and BiophysicsStudies on chemical mechanisms of the action of neurotransmitters and hormones
47 Citations1968Mabel R. Hokin
It is suggested that the increased incorporation of 32 P into phosphatides may represent a second, adaptive response to hormone—part of a gearing up of the cell to meet greater demands for the secretion of zymogens than it had previously been meeting.
Biochemical JournalThe subcellular distribution of 32P-labelled phospholipids, 32P-labelled ribonucleic acid and 125I-labelled iodoprotein in pig thyroid slices. Effect <i>in vitro</i> of thyrotrophic hormone and dibutyryl-3′,5′-(cyclic)-adenosine monophosphate
42 Citations1969P.R. Kerkof, Jamshed R. Tata
Thyrotrophic hormone causes a co-ordinated increase in the formation or accumulation of phospholipids, RNA and iodoprotein associated with the endoplasmic reticulum, and that 6-N-2'-O-dibutyryl-3',5'-(cyclic)-AMP mimics the more rapid effects of thyrotrophic hormones on transport and metabolic functions of thyroid cells, but does not influence their slower biosynthetic responses to the hormone.
Journal of NeurochemistryEFFECTS OF DOPAMINE, GAMMA‐AMINOBUTYRIC ACID AND 5‐HYDROXYTRYPTAMINE ON INCORPORATION OF <sup>32</sup>P INTO PHOSPHATIDES IN SLICES FROM THE GUINEA PIG BRAIN<sup>1</sup>
41 Citations1970Mabel R. Hokin
In eight areas of the guinea pig brain in which the effects of 01 mM‐dopamine were studied, the only significant increase in incorporation of 32P intoosphatides was into phosphatidic acid in the hypothalamus; there was significant inhibition of incorporation of32P into phosph atidylcholine in cerebellar cortex and thalamus, and into phosphorus‐plus‐phosphatidylserine in the olfactory bulbs.
Biochemical JournalEffect of adrenaline on 32P incorporation into rat fat-cell phospholipids
37 Citations1972Janet M. Stein, C. N. Hales
The phospholipid composition of fat-cells prepared from rat epididymal fat-pad was determined and incubation with adrenaline resulted in increased incorporation that was related to the concentration of adrenaline, and the pattern of incorporation after incubation for 1h was determined.
British Journal of PharmacologyEffects on cardiac muscle of the β‐adrenoceptor blocking drugs INPEA and LB46 in relation to their local anaesthetic action on nerve
37 Citations1971Bramah N. Singh, E. M. Vaughan Williams
It is shown that in reducing the maximum rate of depolarization of intracellularly recorded cardiac action potentials, LB46 was 20 times more potent than INPEA and in blocking the effects of isoprenaline on isolated cardiac muscle, there was a net gain of 80 times in the specificity of LB46 for class 2 (antisympathetic) over class 1 (depression of MRD) antidysrhythmic actions.
NatureEffect of Epinephrine on the Metabolism of the Inositol Phosphatides in Rat Heart in vivo
32 Citations1966Zane N. Gaut, C.G. Huggins
Data shows that the “phosphatidepeptide-like” fraction contains inositol mono-, di-, and tri-phosphate, which suggests that this fraction could properly be called a phosphoinositide complex.
The Journal of Cell BiologyTHE EFFECTS OF SECRETAGOGUES ON THE INCORPORATION OF [2-3H]MYOINOSITOL INTO LIPID IN CYTOLOGICAL FRACTIONS IN THE PANCREAS OF THE GUINEA PIG IN VIVO
31 Citations1973Dorothy Gerber, Margaret Davies +1 more
It is concluded that the stimulation of phosphatidylinositol synthesis by pancreatic secretagogues is confined to microsomal elements and does not play any role in membrane flow.
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid MetabolismThe action of adrenalin and glucagon on the metabolism of phospholipids in rat liver
29 Citations1966Gertrudis de Torrontegui, J. Berthet
The results show that not all of the metabolic actions of adrenalin on the liver are shared by glucagon nor, probably, mediated by Ado-3',5'- P.
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism“Phospholipid effect” and secretion in the rat pancreas
21 Citations1972H Bauduin, F. Cantraine
The statistical analysis of the data supports the concept of a “threshold” secretion level above which the phospholipid effect invariably occurs.
Biochemical PharmacologyThe effect of noradrenaline on the incorporation of 32P into brain phospholipids
17 Citations1970J M Sneddon, P. Keen
Rat brain homogenates incubated with noradrenaline for 5 min showed reduced incorporation of 32P into phospholipids, suggesting a possible transmitter role for nor adrenaline in the brain.
American Journal of Physiology-Legacy ContentMetabolism of phosphorus in the supersensitive submaxillary gland of the cat
14 Citations1963Hugh J. Burford, C.G. Huggins
Findings from the supersensitive submaxillary gland of the cat are interpreted as supporting evidence for the participation of these two fractions in secretion of saliva in a manner not yet understood.
Experimental Biology and MedicineElectrical Stimulation and Metabolism of a Phosphoinositide Complex in the Salivary Gland.
8 Citations1966Zane N. Gaut, C. Steffek +1 more