Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis
Infection and ImmunityPublished 1 February 1981Open access
Daniel A. Portnoy, S L Moseley, Stanley Falkow
Citations594
SJR quartileQ1
SJR score1.05
SNIP0.81
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TL;DR
Yersinia enterocolitica isolates harboring a particular species of plasmid deoxyribonucleic acid showed a high degree of lethality for gerbils and caused the detachment of HEp-2 tissue cell monolayers, but invasiveness of HEP-2 cells was shown not to be a plasmids-mediated property.
Abstract
Yersinia enterocolitica isolates harboring a particular species of plasmid deoxyribonucleic acid showed a high degree of lethality for gerbils and caused the detachment of HEp-2 tissue cell monolayers. Strains cured of their plasmid deoxyribonucleic acid showed loss of these properties. However, invasiveness of HEp-2 cells was shown not to be a plasmid-mediated property. The expression of plasmid-associated properties, including at least three major outer membrane polypeptides, occurred during growth at 37 but not at 25 degrees C and was related to the concentration of calcium in the growth medium. The plasmid species associated with these properties ranged in molecular mass from 40 x 10(6) to 48 x 10(6) daltons and comprised a family of related plasmids.
Keywords
Biochemistry, Genetics and Molecular BiologyPharmacology, Toxicology and Pharmaceutics
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