<i>Arabidopsis thaliana</i> Centromere Regions: Genetic Map Positions and Repetitive DNA Structure
Genome ResearchPublished 1 November 1997Open access
Elaine K. Round, Susan K. Flowers, Eric J. Richards
Citations162
SJR quartileQ1
SJR score3.91
SNIP1.37
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Results indicate that the centromere regions of A. thaliana resemble human centromeres in size and genomic organization.
Abstract
The genetic positions of the five Arabidopsis thaliana centromere regions have been identified by mapping size polymorphisms in the centromeric 180-bp repeat arrays. Structural and genetic analysis indicates that 180-bp repeat arrays of up to 1000 kb are found in the centromere region of each chromosome. The genetic behavior of the centromeric arrays suggests that recombination within the arrays is suppressed. These results indicate that the centromere regions of A. thaliana resemble human centromeres in size and genomic organization.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
Proceedings of the National Academy of SciencesGenomic sequencing.
8,227 Citations1984George M. Church, W Gilbert
The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
ScienceSeparation of Large DNA Molecules by Contour-Clamped Homogeneous Electric Fields
1,518 Citations1986Gilbert Chu, Douglas Vollrath +1 more
Electric fields can be manipulated by a method in which multiple electrodes are arranged along a closed contour and clamped to predetermined electric potentials and the pattern of separation is independent of position in the gel, which is an advantage over previous methods.
CellIsolation of a higher eukaryotic telomere from Arabidopsis thaliana
701 Citations1988Eric J. Richards, Frederick M. Ausubel
A method for constructing genomic libraries enriched for telomeric DNA sequences, enabling the isolation of telomeres from higher eukaryotic organisms with large chromosomes, was developed and used to clone telomeral DNA sequences from the flowering plant Arabidopsis thaliana.
Nature GeneticsFormation of de novo centromeres and construction of first-generation human artificial microchromosomes
651 Citations1997John Harrington, Gil Van Bokkelen +3 more
This first-generation system for the construction of human artificial chromosomes should be suitable for dissecting the sequence requirements of human centromeres, as well as developing constructs useful for therapeutic applications.
The Plant JournalRecombinant inbred lines for mapping RFLP and phenotypic markers in <i>Arabidopsis thaliana</i>
571 Citations1993Clare Lister, Caroline Dean
Recombinant inbred lines of Arabidopsis thaliana (Arabidopsis) have been generated from a cross between the ecotypes Landsberg erecta and Columbia, providing the material to map new probes or phenotypic traits polymorphic between Landsberg erection and Columbia.
Trends in GeneticsHierarchical order in chromosome-specific human alpha satellite DNA
515 Citations1987Huntington F. Willard, John S. Waye
Analysis of the sequence organization of these diverged chromosomal subsets provides a framework for considering mechanisms of generation of sequence diversity and for understanding evolutionary processes of DNA family homogenization and polymorphism.
Nucleic Acids ResearchEffect of site-specific modification on restriction endonucleases and DNA modification methyltransferases
425 Citations1994Michael McClelland, Michael Nelson +1 more
A table of known site-specific DNA modification methyltransferases and their specificities is presented along with EMBL database accession numbers for cloned genes.
ScienceThe Centromere: Hub of Chromosomal Activities
358 Citations1995Ann F. Pluta, Alastair M. Mackay +3 more
Point centromeres, found in the budding yeasts, are compact loci whose constituent proteins are now beginning to yield to biochemical analysis and have an important role in checkpoint regulation during mitosis.
Molecular and General Genetics MGGThe DNA of Arabidopsis thaliana
313 Citations1984Leslie Sise Leutwiler, Barbara R. Hough‐Evans +1 more
It is demonstrated that Arabidopsis has an extraordinarily small haploid genome size and a low level of cytosine methylation for an angiosperm, and appears to have little repetitive DNA in its nuclear DNA, in contrast to other higher plants.
Molecular and General Genetics MGGA highly repeated DNA sequence in Arabidopsis thaliana
267 Citations1986José M. Martı́nez-Zapater, Mark Estelle +1 more
Three members of a family of highly repeated DNA sequences from Arabidopsis thaliana have been cloned and characterized and several regions of homology and a similar length of the repeat unit are found.
Trends in GeneticsCentromeres of mammalian chromosomes
248 Citations1990Huntington F. Willard
Data from direct functional assays of chromosome segregation are most consistent with a structural and possibly functional role for satellite DNA in the mammalian centromere.
The Plant JournalLocalization of tandemly repeated DMA sequences in <i>Arabidopsis thaliana</i>
243 Citations1991Jolanta Małuszyńska, J. S. Heslop‐Harrison
In-situ hybridization to interphase nuclei and chromosomes of Arabidopsis thaliana shows that there are four sites of rDNA in a diploid nucleus, and the strength of hybridization indicates that copy number is similar at both pairs of sites.
Proceedings of the National Academy of SciencesLong-range organization of tandem arrays of alpha satellite DNA at the centromeres of human chromosomes: high-frequency array-length polymorphism and meiotic stability.
228 Citations1989Rachel Wevrick, H.F. Willard
High-frequency array length variation and the potential to detect meiotic recombination within them allow direct comparisons of genetic and physical distances in the region of the centromeres of human chromosomes.
GeneticsMolecular characterization of a maize B chromosome centric sequence.
226 Citations1993M. R. Alfenito, James A. Birchler
Cytological mapping using B chromosome derivatives and in situ hybridization show that the B specific repeats are derived from the centric region of the chromosome.
SciencePhysical Map and Organization of <i>Arabidopsis thaliana</i> Chromosome 4
208 Citations1995Renate Schmidt, Joanne West +6 more
The EMBO JournalChromosome walking shows a highly homologous repetitive sequence present in all the centromere regions of fission yeast
189 Citations1986Yukinobu Nakaseko, Yasuhisa Adachi +3 more
The structures in or surrounding the centromeres of S. pombe appear to be much more complex than those of the budding yeast Saccharomyces cerevisiae, which has three chromosomes.
CellLocalization of centromere function in a drosophila minichromosome
184 Citations1995Terence D. Murphy, Gary H. Karpen
It is proposed that the essential core of the centromere is the site of kinetochore formation and that flanking DNA provides two functions: sister chromatid cohesion and indirect assistance in kinetchore formation or function.
CellIntegration of human α-satellite DNA into simian chromosomes: Centromere protein binding and disruption of normal chromosome segregation
182 Citations1992Thomas Haaf
These experiments suggest that alpha-satellite DNA provides the primary sequence information for centromere protein binding and for at least some functional aspect(s) of a mammalian centromeres, playing a role either in kinetochore formation or in sister chromatid apposition.
Trends in GeneticsCentromeres of budding and fission yeasts
178 Citations1990Louise Clarke
Fission yeast provides an excellent model system for studying the role of repeated DNA sequences in centromere function, and a portion of the repeated sequences is organized into a large inverted repeated structure in the Centromere region of each S. pombe chromosome.
The Plant JournalIsolation and mapping of a new set of 129 RFLP markers in <i>Arabidopsis thaliana</i> using recombinant inbred lines
170 Citations1996Yao‐Guang Liu, Norihiro Mitsukawa +3 more
A new collection of 129 Arabidopsis thaliana RFLP markers has been established based upon DNA fragments cloned in the pUC119 plasmid vector and insert end sequences of P1 clones, suggesting that deletions are not rare in A. thalianA.
Journal of Molecular BiologyStructure of the major block of alphoid satellite DNA on the human Y chromosome
161 Citations1987Chris Tyler‐Smith, William R. Brown
The results provide a basis for further structural analysis of the human Y chromosome centromeric region, and suggest that long-range structural polymorphisms of tandemly repeated sequence families may be frequent.
The Plant JournalAn integrated genetic/RFLP map of the <i>Arabidopsis thaliana</i> genome
150 Citations1993Brian M. Hauge, Susan Hanley +9 more
An integrated genetic/restriction fragment length polymorphism (RFLP) linkage map of the nuclear genome of the flowering plant Arabidopsis thaliana is assembled and provides a framework for building an increasingly accurate and useful map.
Nucleic Acids ResearchStructural instability of human tandemly repeated DNA sequences cloned in yeast artificial chromosome vectors
142 Citations1990David L. Neil, Alfredo Villasanté +4 more
The use of rad52 hosts for future library construction is recommended, but some sequences will still be unstable, but DYZ1/DYZ2 or DYz3 containing YACs were more stable.
Proceedings of the National Academy of SciencesDetection of restriction fragment length polymorphisms at the centromeres of human chromosomes by using chromosome-specific alpha satellite DNA probes: implications for development of centromere-based genetic linkage maps.
128 Citations1986H.F. Willard, John S. Waye +4 more
Since the strategy is a general one, the alpha satellite family of DNA should provide a rich source of molecular variation in the human genome and should contribute to the development of centromere-based genetic linkage maps of human chromosomes.
Nucleic Acids ResearchThe centromere region of<i>Arabidopsis thaliana</i>chromosome 1 contains telomere-similar sequences
126 Citations1991Eric J. Richards, Howard M. Goodman +1 more
The structure of an Arabidopsis thaliana genomic clone containing two classes of repetitive DNA elements derived from the centromere region of chromosome 1 is described, one of which is comprised of tandem arrays of a highly reiterated repeat containing degenerate telomere sequence motifs.
The Plant JournalPhysical mapping of the 5S ribosomal RNA genes in <i>Arabidopsis thaliana</i> by multi‐color fluorescence <i>in situ</i> hybridization with cosmid clones
121 Citations1997Minoru Murata, J. S. Heslop‐Harrison +1 more
The 5S ribosomal RNA genes were mapped to mitotic chromosomes of Arabidopsis thaliana by fluorescence in situ hybridization (FISH) and used as a probe to identify chromosomes and arms carrying the 5S rDNA.
GenomicsMouse minor satellite DNA genetically maps to the centromere and is physically linked to the proximal telomere
121 Citations1991David Kipling, Helen E. Ackford +2 more
A long-range physical analysis of these regions in the mouse indicates that many mouse chromosomes are truly telocentric.
GeneticsIslands of complex DNA are widespread in Drosophila centric heterochromatin.
118 Citations1995Mong-Huong Le, Deborah Duricka +1 more
Pulsed-field Southern analysis demonstrated that Drosophila heterochromatin in general is composed of alternating blocks of complex DNA and simple satellite DNA and the presence of a retroposon is demonstrated.
Nature GeneticsLocalization of DNA sequences required for human centromere function through an analysis of rearranged Y chromosomes
116 Citations1993Chris Tyler-Smith, Rebecca J. Oakey +8 more
Alphoid DNA is a functional part of the Y chromosome centromere and two suppressed Y centromeres on translocation chromosomes and dicentric isochromosomes that were also analysed two showed deletions one of which only removed alphoidDNA.
The EMBO JournalMisdivision analysis of centromere structure in maize.
109 Citations1996Étienne Kaszás, James A. Birchler
Results demonstrate that the B repeat is part of the functional centromere, that it is spread throughout its length, and that plant centromeres are composed of repeat units that can be significantly changed in copy number without a change in function.
Genome ResearchA physical map of chromosome 2 of Arabidopsis thaliana.
99 Citations1996Eve Ann Zachgo, M L Wang +7 more
A yeast artificial chromosome (YAC) physical map of chromosome 2 of Arabidopsis thaliana has been constructed by hybridization of 69 DNA markers and 61 YAC end probes to gridded arrays of YAC clones to provide the means for immediately identifying the YACs containing a genetic locus mapped onArabidopsis chromosome 2.
Human Molecular GeneticsDissecting the centromere of the human Y chromosome with cloned telomeric DNA
96 Citations1994K. E. Brown, Michael A. Barnett +4 more
The results demonstrate that chromosomes of this size are capable of accurate segregation and also demonstrate that additional sequences may be required for orderly segregation.
The Japanese Journal of GeneticsCentromeric repetitive sequences in Arabidopsis thalitana.
95 Citations1994Minoru Murata, Yutaka Ogura +1 more
The results suggest that the repetitive sequences of this family were derived from the regions very close to the centromeres or on the centromeric regions themselves.
Human Molecular Genetics<i>De novo</i> formation of several features of a centromere following introduction of a Y alphoid YAC into mammalian cells
94 Citations1994Zoia Larin, Mark D. Fricker +1 more
This work suggests that a few hundred kb of alphoid DNA is sufficient to reconstitute several important features of a centromere, including a cytogenetically visible constriction, CREST antiserum binding and disruption of anaphase chromosome movement.
GeneticaDNA regions flanking the major Arabidopsis thaliana satellite are principally enriched in Athila retroelement sequences
93 Citations1996T. P�lissier, Sylvie Tutois +3 more
An analysis of Arabidopsis thaliana heterochromatic regions revealed that genomic sequences immediately flanking the major 180 bp satellite are essentially made of middle repetitive sequences and that most of these sequences correspond to defective Athila retroelements.
Journal of Molecular BiologyGenomic analysis of sequence variation in tandemly repeated DNA
91 Citations1990Peter E. Warburton, Huntington F. Willard
These analyses show that alpha-satellite arrays are characterized by the presence of localized homogeneous domains containing only one distinct type of repeat unit, which may correspond to transition states in the evolution of tandem multicopy DNA families.
Nucleic Acids ResearchCharacterization of highly repetitive sequences of<i>Arabidopsis thaliana</i>
84 Citations1988C. Simoens, J. Gielen +2 more
Three classes of highly repetitive DNA sequences of Arabidopsis thaliana, composed of tamdemly repeated units of 180, 500, and 160 bp, are analyzed and there is no significant similarity with previously published repetitive elements.
Nucleic Acids ResearchPhysical map of the centromeric region of human chromosome 7: relationship between two distinct alpha satellite arrays
77 Citations1991Rachel Wevrick, Huntington F. Willard
A long-range physical map of the centromeric region of human chromosome 7 has been constructed in order to define the region containing sequences with potential involvement in centromere function and provides a framework for analysis of the structure of DNA in the Centromeric regions of other human and mammalian chromosomes.
Chromosome ResearchMultiple repetitive DNA sequences in the paracentromeric regions of Arabidopsis thaliana L.
75 Citations1997Andrea Brandes, H L Thompson +2 more
The results show that the paracentromeric heterochromatin of A. thaliana chromosomes is composed not only of the tandemly arranged 180-bp repeat family pAL1/pAtMr, but also of some other repetitive sequences, thus giving a better understanding of the organization of sequences at the centromeres of the genome.
ChromosomaMouse centromere mapping using oligonucleotide probes that detect variants of the minor satellite
73 Citations1994David Kipling, Helen Wilson +3 more
The existence of chromosome-specific minor satellite sequences implies that the rate of sequence exchange between non-homologous chromosomes relative to the rate between homologous chromosome is much lower than has been postulated.
The Plant JournalUse of RFLPs larger than 100 kbp to map the position and internal organization of the nucleolus organizer region on chromosome 2 in <i>Arabidopsis thaliana</i>
57 Citations1995Gregory P. Copenhaver, Jed H. Doelling +2 more
Molecular and Cellular BiologyCloning and Characterization of Centromeric DNA from <i>Neurospora crassa</i>
53 Citations1994Michael Centola, John Carbon
The predominantly transitional differences between copies of the centromere-specific sequence repeats and their high A+T content suggest that their divergence was mediated by repeat-induced point (RIP) mutations.
Nucleic Acids ResearchIdentification and distribution of seven classes of middle-repetitive DNA in the Arabidopsis thaliana genome
49 Citations1996H L Thompson
Seven novel repetitive sequences have been identified and characterized and sequences hybridizing to 106B, 164A and 163A were found exclusively at the centromeric region of chromosome 4, relative to other repeated sequence families and single copy sequences.
GeneticaTrisomics inArabidopsis thaliana and the location of linkage groups
48 Citations1983Maarten Koornneef, J.H. van der Veen
Theer/er genotype is found to have a pronounced adverse effect on female fertility of the trisomics and the higher male transmission rates were found among the telotrisomics.
Journal of Molecular BiologyStructure of the Sequences Adjacent to the Centromeric Alphoid Satellite DNA Array on the Human Y Chromosome
44 Citations1993Katrina F. Cooper, Richard B. Fisher +1 more
Eighteen yeast artificial chromosome (YAC) clones containing alphoid satellite DNA and adjacent sequences from the human Y chromosome have been identified from three different YAC libraries.
Mammalian GenomeCytological and molecular characterization of centromeres in Mus domesticus and Mus spretus
43 Citations1992Sandya Narayanswami, Norman A. Doggett +4 more
Although both species possess all three sequences, except for the telomeres, their distribution relative to one another is not conserved, and preliminary molecular maps of the centromere regions of Mus domesticus and Mus spretus are proposed.
Molecular and Cellular BiologyCloning and characterization of centromeric DNA from Neurospora crassa.
40 Citations1994M Centola, J Carbon
Molecular and Cellular BiologyChromosome instability associated with human alphoid DNA transfected into the Chinese hamster genome.
38 Citations1988Michael W. Heartlein, Joan H.M. Knoll +1 more
Molecular and General Genetics MGGA novel repetitive sequence associated with the centromeric regions of Arabidopsis thaliana chromosomes
35 Citations1996H L Thompson, Renate Schmidt +3 more
A novel repetitive sequence cloned in the plasmid mi167, and present in ∼90 copies in the genome of Arabidopsis thaliana ecotype Columbia is described, which supports the idea that the majority of middle repetitive DNA in theArabidopsis genome is clustered.
Cold Spring Harbor Monograph Archive2 Structure and Organization of the Arabidopsis thaliana Nuclear Genome
32 Citations1994Elliot M. Meyerowitz
The nuclear genome of Arabidopsis thaliana is unusually small for a flowering plant and has remarkably little dispersed repetitive DNA, which facilitates a series of different types of experiments in molecular genetics and has allowed facile cloning of manyArabidopsis genes by methods that would be difficult or impossible if the genome were larger or more typical in its content of repetitive sequences.
Nucleic Acids ResearchThe organisation of repetitive sequences in the pericentromeric region of human chromosome 10
28 Citations1993Michael S. Jackson, Predrag Slijepčević +1 more
Pulsed field gel electrophoresis demonstrates that three satellite DNA families present in the pericentromeric region of chromosome 10 are organised into five discrete arrays which are linked within a region of approximately 5.3 Megabases (Mb) of DNA.
GeneticaThe use of telotrisomics for centromere mapping in Arabidopsis thaliana (L.) Heynh.
14 Citations1983Maarten Koornneef
For 4 different telotrisomics of Arabidopsis thaliana segregation ratios are given for a number of genes located on the extra telocentric chromosome, and the centromere position may be determined.