TLP1: A Gene Encoding a Protein Component of Mammalian Telomerase Is a Novel Member of WD Repeats Family
CellPublished 1 March 1997Open access
Jun‐ichi Nakayama, Motoki Saito, Hideo Nakamura, Akira Matsuura, Fuyuki Ishikawa
Citations379
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
The rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80, encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230, which were cloned and characterized and showed that p240 is modified to p230 in vivo.
Abstract
We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240 and p230 were copurified with telomerase activity in a series of extensive purification experiments. These results strongly suggest that the TLP1 proteins are components of, or are closely associated with, the rat telomerase. A pulse-chase experiment showed that p240 is modified to p230 in vivo. p230 was the dominant form in telomerase-positive cells, suggesting that modification of the TLP1 protein may regulate telomerase activity in vivo.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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