Mutations in Methanobacterium formicicum conferring resistance to anti-80S ribosome-targeted antibiotics
Molecular and General Genetics MGGPublished 1 March 1985
H. Hummel, August Böck
Citations19
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TL;DR
It appears therefore, that the anisomycin interaction sites on the 70S ribosomes from M. formicicum bear the structural features typical of eukaryotic 80S organelles.
Abstract
Mutants of Methanobacterium formicicum resistant to the anti-80S ribosome-targeted inhibitor anisomycin were isolated and characterized. The resistance phenotype is correlated with a mutationally altered 50S ribosomal subunit. Anisomycin resistance in the mutants is accompanied by cross-resistance to other inhibitors of the 80S peptidyl-transferase centre like narciclasine, bruceantin, trichodermin and verrucarin A and by hypersensitivity to sparsomycin. This phenotype is identical to that reported for anisomycin-resistant mutants of yeast; it appears therefore, that the anisomycin interaction sites on the 70S ribosomes from M. formicicum bear the structural features typical of eukaryotic 80S organelles.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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