Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32
Biochemical JournalPublished 1 April 1996Open access
Elizabeth A. Slee, Huijun Zhu, Sek C. Chow, Marion MacFarlane, Donald W. Nicholson, Gerald M. Cohen
Citations495
SJR quartileQ1
SJR score2.06
SNIP0.98
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TL;DR
It is demonstrated that benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK), an ICE-like protease inhibitor, inhibits apoptosis by preventing the processing of CPP32 to its active form, suggesting that novel inhibitors of apoptosis can be developed which prevent processing of proforms of ICE- like proteases.
Abstract
Interleukin-1 beta converting enzyme (ICE)-like proteases, which are synthesized as inactive precursors, play a key role in the induction of apoptosis. We now demonstrate that benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK), an ICE-like protease inhibitor, inhibits apoptosis by preventing the processing of CPP32 to its active form. These results suggest that novel inhibitors of apoptosis can be developed which prevent processing of proforms of ICE-like proteases.
Keywords
Biochemistry, Genetics and Molecular Biology
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