Expression of the c- <i>fos</i> Gene and of an <i>fos</i> -Related Gene Is Stimulated by Platelet-Derived Growth Factor
SciencePublished 30 November 1984
Brent Cochran, John Zullo, Inder M. Verma, Charles D. Stiles
Citations447
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
Under low stringency conditions, the mouse v-fos gene cross-hybridizes with the PDGF-inducible complementary DNA clone, however, the messenger RNA transcripts of mouse c- fos and the new fos-related gene can be distinguished by gel electrophoresis and by S1 nuclease analysis.
Abstract
Complementary DNA clones of genes induced by platelet-derived growth factor (PDGF) in BALB/c-3T3 cells were isolated; one such clone contains a domain having nucleotide sequence homology with the third exon of c-fos. This nucleotide sequence homology is reflected in the predicted amino acid sequences of the gene products. Under low stringency conditions, the mouse v-fos gene cross-hybridizes with the PDGF-inducible complementary DNA clone. However, the messenger RNA transcripts of mouse c-fos and the new fos-related gene can be distinguished by gel electrophoresis and by S1 nuclease analysis. Expression of the authentic c-fos gene is induced by PDGF and superinduced by the combination of PDGF and cycloheximide.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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