Changes in immediate early gene expression during postnatal development of cat cortex and cerebellum
Molecular Brain ResearchPublished 1 January 1992
Matthew McCormack, Kenneth M. Rosen, Lydia Villa‐Komaroff, George D. Mower
Citations60
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TL;DR
The results suggest that temporal changes in the combinatorial interaction of different IEGs during early postnatal life are important for normal brain development.
Abstract
Postnatal brain development involves interactions between extracellular signals and preprogrammed genetic events. Immediate early genes (IEGs) are a group of genes that are induced by extracellular signals and their protein products alter transcription by binding regulatory elements in other genes. Using Northern and slot blot analysis of total RNA isolated from visual cortex, frontal cortex, and cerebellum of cats, we have determined the postnatal development patterns of mRNA expression for 5 of these genes, c-fos, erg-1, c-jun, jun-B, and c-myc. Each gene had a distinct developmental pattern of mRNA expression, and for a given gene, these patterns were often different in different brain structures. These results suggest that temporal changes in the combinatorial interaction of different IEGs during early postnatal life are important for normal brain development.
Keywords
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Molecular Cloning: A Laboratory Manual 2
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Analytical BiochemistrySingle-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
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A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
ScienceTranscriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins
3,238 Citations1989Pamela J. Mitchell, Robert Tjian
This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
The Journal of PhysiologyThe period of susceptibility to the physiological effects of unilateral eye closure in kittens
3,029 Citations1970David H. Hubel, T. N. Wiesel
Kittens were visually deprived by suturing the lids of the right eye for various periods of time at different ages to study the effect of monocular eye closure on the number of cells that can be influenced by the previously closed eye.
CellPhorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor
3,021 Citations1987Peter Angel, Masayoshi Imagawa +7 more
Results strongly suggest that AP-1 is at the receiving end of a complex pathway responsible for transmitting the effects of phorbol ester tumor promoters from the plasma membrane to the transcriptional machinery.
NeuronThe regulation and function of c-fos and other immediate early genes in the nervous system
2,456 Citations1990Morgan Sheng, Michael E. Greenberg
This review highlights the importance of identifying the genes that are responsive to trans-synaptic stimulation and membrane electrical activity in neural cells and proposes that IEGs encode regulatory proteins that control the expression of late response genes.
CellPurified transcription factor AP-1 interacts with TPA-inducible enhancer elements
2,043 Citations1987Wes Lee, Pamela J. Mitchell +1 more
It is demonstrated that multiple synthetic copies of the consensus AP-1-binding site can act as TPA-inducible enhancers in various plasmid constructs after transfection into HeLa cells, suggesting that AP- 1 is a transcription factor that functions by interacting with a specific enhancer element, and that its activities may be modulated by treatment of cells with TPA.
ScienceExpression of c- <i>fos</i> Protein in Brain: Metabolic Mapping at the Cellular Level
1,931 Citations1988Stephen M. Sagar, Frank R. Sharp +1 more
Fos immunohistochemistry provides a cellular method to label polysynaptically activated neurons and thereby map functional pathways in response to polysynaptic activation.
Nucleic Acids ResearchIsolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene
1,827 Citations1985J. Yun Tso, Xiao-Hong Sun +3 more
Comparison of these two cDNA sequences with those of the chicken, Drosophila and yeast genes allows the analysis of the evolution of the GAPDH genes in detail.
ScienceMapping Patterns of c- <i>fos</i> Expression in the Central Nervous System After Seizure
1,811 Citations1987James I. Morgan, Donna Cohén +2 more
A dramatic and specific induction of c-fos was observed in identifiable neuronal populations in vivo after administration of the convulsant Metrazole, and this effect was time- and dose-dependent and was abolished by prior treatment with the anticonvulsant drugs diazepam or pentobarbital.
CellThe c-fos protein interacts with c-JunAP-1 to stimulate transcription of AP-1 responsive genes
1,360 Citations1988Robert Chiu, William J. Boyle +4 more
Fos is a trans-acting factor that is capable of stimulating gene expression not by direct binding to DNA but by interaction with the sequence-specific transcription factor AP-1, therefore recognition of specific cis-elements by AP- 1 is a prerequisite for Fos-mediated stimulation of gene expression.
CellA zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization
1,291 Citations1988Vikas P. Sukhatme, Xinmin Cao +10 more
Sequence analysis of murine Egr-1 cDNA predicts a protein with three DNA binding zinc fingers, and results suggest that EGR1 may function as a transcriptional regulator in diverse biological processes.
Annual Review of BiochemistryEUKARYOTIC TRANSCRIPTIONAL REGULATORY PROTEINS
1,263 Citations1989Peter F. Johnson, Steven L. McKnight
Binding Proteins, ClEBP and OBPlOO, 8 1 3 Glucocorticoid Rece ptor, 9 1 3 HAPl, and 8 1 1 0.
Developmental Neurobiology
1,220 Citations1978Marcus Jacobson
The neurobiology of autism, aggression understanding and preventing violence, and the construction of the self developmental and sociocultural foundations are reviewed.
ScienceConcurrent Overproduction of Synapses in Diverse Regions of the Primate Cerebral Cortex
1,142 Citations1986Pasko Rakić, Jean‐Pierre Bourgeois +3 more
This isochronic course of synaptogenesis in anatomically and functionally diverse regions indicates that the entire cerebral cortex develops as a whole and that the establishment of cell-to-cell communication in this structure may be orchestrated by a single genetic or humoral signal.
Cellc-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinities
1,065 Citations1988Thanos D. Halazonetis, Katia Georgopoulos +2 more
Journal of Biological ChemistryNerve growth factor and epidermal growth factor induce rapid transient changes in proto-oncogene transcription in PC12 cells.
890 Citations1985Michael E. Greenberg, Lloyd A. Greene +1 more
The results suggest that c-fos, c-myc, and actin induction may be general nuclear responses to growth or differentiation factors in a variety of different cell types.
CellDNA binding activities of three murine Jun proteins: Stimulation by Fos
818 Citations1988Yusaku Nakabeppu, Kevin Ryder +1 more
The general conclusion is that c-Jun, Jun-B, and Jun-D are similar in their DNA binding properties and in their interaction with Fos, and if there are functional differences between them, they are likely to involve other activities of the Jun proteins.
CellThe human c-myc oncogene: Structural consequences of translocation into the igh locus in Burkitt lymphoma
736 Citations1983Jim Battey, Christopher Moulding +6 more
The structure of the translocated gene and the nature of its linkage to the immunoglobulin locus and the presence of two c-myc promoters and consequently two long leader sequences raise novel possibilities for the activation of an oncogene.
Proceedings of the National Academy of SciencesA gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.
733 Citations1988B A Christy, Lester F. Lau +1 more
It is reported that the cDNA derived from one of these immediate early genes (zif/268) encodes a protein with three tandem "zinc finger" sequences typical of a class of eukaryotic transcription factors.
The Journal of PhysiologyOcular dominance in layer IV of the cat's visual cortex and the effects of monocular deprivation.
701 Citations1978Carla J. Shatz, Michael P. Stryker
The findings suggest that the thalamocortical projection is physically rearranged as a consequence of monocular deprivation, as has been demonstrated for layer IVc of the monkey's visual cortex.
CellJun-B differs in its biological properties from, and is a negative regulator of, c-Jun
700 Citations1989Robert Chiu, Peter Angel +1 more
Using chimeric proteins, it is shown that the distinct behavior of c-Jun and Jun-B is due to differences in their activation domains, which greatly expands the regulatory potential of the Jun family.
The Journal of PhysiologyInnate and environmental factors in the development of the kitten's visual cortex.
692 Citations1975Colin Blakemore, Richard C. Van Sluyters
A study of the receptive fields of 771 cells recorded in the visual cortex of twenty‐five kittens reared normally or subjected to various kinds of visual deprivation or environmental manipulation finds that in deprived animals, there are a number of genuinely orientation selective cells.
The Journal of Comparative NeurologyOcular dominance columns and their development in layer IV of the cat's visual cortex: A quantitative study
668 Citations1978Simon LeVay, Michael P. Stryker +1 more
The distribution of geniculocortical afferents serving the left and right eyes was studied in abult cats and in kittens of various ages.
CellCommon DNA binding site for Fos protein complexesand transcription factor AP-1
614 Citations1988Frank J. Rauscher, Lidia Sambucetti +3 more
It is shown that there is a dramatic increase in the FSE2 binding complex when Fos levels are induced with serum, benzodiazepine, and nerve growth factor or are expressed from a v-fos gene, suggesting a functional relationship between Fos and AP-1.
Proceedings of the National Academy of SciencesTwo mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells.
579 Citations1988Patrick Lemaire, O Revelant +2 more
These properties suggest that Krox-20 andKrox-24 may encode transcription factors with identical DNA target sequences and that these factors may be involved in the modulation of cell proliferation and differentiation.
NatureDifferential expression of myc family genes during murine development
548 Citations1986Kathryn A. Zimmerman, George D. Yancopoulos +10 more
It is reported that high-level expression of N- and L-myc is very restricted with respect to tissue and stage in the developing mouse, while that of c- myc is more generalized, suggesting that differential myc gene expression has a role in mammalian development and that the normal expression patterns of these genes generally predict the types of tumours in which they are expressed or activated.
The Journal of Comparative NeurologyThe development of synapses in the visual system of the cat
524 Citations1975B.G. Cragg
The gradual separation of neurones by neuropil during development precedes a parallel increase in the density of synapses by about one week, which rises to a peak of about 13,000 at seven weeks after birth and falls to slightly lower values in adult cats as the glial cells continue to develop.
Genes & DevelopmentGrowth factors and membrane depolarization activate distinct programs of early response gene expression: dissociation of fos and jun induction.
503 Citations1989David P. Bartel, Morgan Sheng +2 more
It is concluded that multiple nonconvergent signal transduction pathways control early response gene expression and that the diversity and specificity of cellular response to environmental change can be accounted for by the differential combinatorial induction of a relatively small number of early response genes.
Journal of NeuroscienceStudies of the earliest generated cells of the cat's visual cortex: cogeneration of subplate and marginal zones
495 Citations1985MB Luskin, CJ Shatz
A comparison between the number of early generated cells found in fetal and newborn brains with that found in adult brains suggests that these cells are generated initially in substantial numbers but then largely disappear during early postnatal life, since injections of [3H]thymidine between E24 and E30 yielded large numbers of labeled cells in the white matter and layer 1 at birth, but very few at 2 months postnatal.
Cellfos-associated cellular p39 is related to nuclear transcription factor AP-1
492 Citations1988Paolo Sassone‐Corsi, William W. Lamph +2 more
It is shown that one of the fos-associated proteins, p39, is immunologically and structurally related to nuclear factor AP-1, and the sequence TGACTCA, which is the consensus minimal binding site of AP- 1, is also recognized by gel-purified p39.
Celljun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun
487 Citations1989J. Schütte, Jean Viallet +4 more
It is demonstrated that, whereas jun-B and c-jun each participate in AP-1 trans-activation and malignant transformation, interactions between them involve negative regulation.
Proceedings of the National Academy of SciencesInduction of protooncogene c-jun by serum growth factors.
438 Citations1988Kevin Ryder, Daniel Nathans
Using jun-B as a probe, a cDNA is isolated that is the murine version of the protooncogene c-jun, which encodes the mammalian transcription factor AP-1 and is likely to play a role in regulating the genetic program induced by growth factors.
The Journal of PhysiologyThe effect of visual experience on the development of stimulus specificity by kitten cortical neurones
394 Citations1974John D. Pettigrew
Proceedings of the National Academy of SciencesConvulsant-induced increase in transcription factor messenger RNAs in rat brain.
379 Citations1988David Saffen, Andrew J. Cole +4 more
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280 Citations1980Carl R. Olson, R. D. Freeman
It was found that the effectiveness of monocular deprivation rose prior to postnatal day 28, remained high through day 48 and then subsided gradually, probably persisting at least through the end of the fourth postnatal month.
The Journal of PhysiologyEarly development of visual cortical cells in normal and dark‐reared kittens: relationship between orientation selectivity and ocular dominance.
265 Citations1978Yves Frégnac, M. Imbert
The results confirm that cells having the same orientation‐specific response properties as adult cortical visual neurones are present in the earliest stages of post‐natal development, independently of visual experience, however, to maintain and develop these specific cells after the third week of post-natal life, visual experience is necessary.
The Journal of Cell BiologyN-myc proto-oncogene expression during organogenesis in the developing mouse as revealed by in situ hybridization.
194 Citations1988Gabriele Mugrauer, Frederick W. Alt +1 more
In situ hybridization of tissue sections is demonstrated to demonstrate a restricted expression of N-myc mRNA to a few tissues and to areas where the first differentiation stages occur, raising the possibility that N- myc may participate in the control of these early differentiation events.
CellTrans-repression of the mouse c-fos promoter: A novel mechanism of fos-mediated trans-regulation
184 Citations1989Frances C. Lucibello, Carolin Lowag +2 more
It is suggested that trans-repression of the type described here is neither sufficient nor required for Fos-induced transformation and that Fos mutants with an impaired leucine zipper are defective in trans- repression and transformation.
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171 Citations1989David G. Wilkinson, Sangita Bhatt +2 more
It is suggested that c-jun and junB have distinct, tissue-specific roles in cell proliferation and differentiation during Fetal growth and organogenesis in the mouse by in situ hybridization analysis of their expression patterns.
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131 Citations1989Peter Schmid, Wolfgang A. Schulz +1 more
The c- myc protooncogene in mouse embryos was shown by RNA in situ hybridization to be preferentially expressed in tissues of endodermal and mesodermal origin, and the thymus represented the only hematopoietic organ with high c-myc expression.
Developmental Brain ResearchThe postnatal development of synapses in the different laminae of the visual cortex in the normal kitten and in kittens with eyelid suture
113 Citations1983D.A. Winfield
Much of the changes in the development of synapses involved asymmetric axo-spinous synapses, with a small reduction in the number of symmetric synapses chiefly in lamina IV.
PubMedOptimizing the northern blot procedure.
109 Citations1990Kenneth M. Rosen, E D Lamperti +1 more
Methods for preparing formaldehyde-agarose gels for use in Northern blotting which yield consistent high quality results are described and tested in seven different commercially available membranes in Northern blots.
Genes & DevelopmentA FOS protein is present in a complex that binds a negative regulator of MYC.
106 Citations1989Nissim Hay, Masato Takimoto +1 more
It is reported that a negative regulator of the human proto-oncogene MYC comprises no more than 26 bp of DNA, with sequence that resembles the regulators of at least two other genes, and nuclear factors that interact with the regulator are described.
The EMBO JournalProto‐oncogene c‐myc is expressed in cerebellar neurons at different developmental stages.
96 Citations1986C. Ruppert, Dan Goldowitz +1 more
It is concluded that neurons in vivo can accumulate c‐myc messenger during proliferation and/or differentiation, perhaps as a cellular response to an external signal.
Molecular Brain ResearchVariations in c-fos gene expression during rat brain development
74 Citations1988Ruth M. Gubits, Jeanette L. Hazelton +1 more
The results demonstrate that very low levels of c-fos mRNA are detectable during the period of development characterized by rapid mitosis, whereas much higher concentrations are found in the brains of older neonatal animals and adults.
The Journal of Comparative NeurologyPostnatal changes in the distribution of acetylcholinesterase in kitten striate cortex
72 Citations1985Mark F. Bear, Kenneth M. Carnes +1 more
The postnatal development of axons and cells in kitten striate cortex that contain acetylcholinesterase (AChE) are traced by using a modification of Koelle's histochemical method.
Molecular and Cellular Biologyc<i>-fos</i> Proto-Oncogene Expression in the Nervous System during Mouse Development
58 Citations1989Jean-François Caubet
It is shown, by in situ hybridization, that c-fos is expressed in the nervous system during mouse development, and this expression was found to be restricted to specific regions at late stages of development, particularly to the spinal cord, dorsal root ganglia, and olfactory lobe.
Proceedings of the National Academy of Sciencespp60c-src expression in the developing rat brain.
57 Citations1988Christine A. Cartwright, Rabi Simantov +3 more
The results are consonant with the idea that pp60c-src is the product of a developmentally regulated gene that is important for the differentiation and/or the continuing function of neurons.
Molecular and Cellular Biologyc-fos proto-oncogene expression in the nervous system during mouse development.
56 Citations1989J F Caubet
The Journal of Comparative NeurologyQuantitative comparisons of gamma‐aminobutyric acid neurons and receptors in the visual cortex of normal and dark‐reared cats
44 Citations1988George D. Mower, Robin Rustad +1 more
Whether visual input is necessary for the normal postnatal anatomical development of the GABA system by comparing GABA neurons and receptors in the visual cortex of normal and dark‐reared cats is asked.
Experimental Brain ResearchThe laminar distribution and postnatal development of serotonin-immunoreactive axons in the cat primary visual cortex
27 Citations1990Qiang Gu, Bhavika B. Patel +1 more
The laminar distribution and postnatal development of profiles immunoreactive to antibodies directed against serotonin (5-hydroxytryptamine, 5-HT) have been investigated in the primary visual cortex of cats.
The Journal of PhysiologySynaptic frequency response: the influence of sinusoidal changes in stimulation frequency on the amplitude of the end‐plate potential
4 Citations1973E. M. Landau, Y. Lass
The synaptic response to frequency modulated trains of stimuli was investigated at the neuromuscular junction of the frog and the frequency of stimulation usually varied sinusoidally about a carrier frequency of ca.