Designed evolution of enzymatic properties
Current Opinion in BiotechnologyPublished 1 August 2000
Ioanna P. Petrounia, Frances H. Arnold
Citations163
SJR quartileQ1
SJR score1.86
SNIP1.50
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Abstract
By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis. Recent advances include the evolution of a novel catalytic activity using the α/β barrel scaffold, evolution of a cofactor-free monooxygenase, and the engineering of regulatable enzymes. New screening systems for enantioselectivity and protein solubility, and the continuing stream of new methods for creating enzyme libraries further extend evolution’s reach.
Keywords
Biochemistry, Genetics and Molecular BiologyEngineering
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