Simple procedure for distinguishing CCC, OC, and L forms of plasmid DNA by agarose gel electrophoresis
PlasmidPublished 1 May 1981
Gilberto Hintermann, Hans‐Martin Fischer, Reto Crameri, R. Hütter
Citations149
SJR quartileQ3
SJR score0.60
SNIP0.46
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TL;DR
A simple and rapid method discriminating between covalently closed circular (CCC), open circular (OC), and linear (L) forms of plasmid DNA is presented.
Abstract
A simple and rapid method discriminating between covalently closed circular (CCC), open circular (OC), and linear (L) forms of plasmid DNA is presented. Two consecutive steps of agarose gel electrophoresis with a single DNA sample are used; use is made between the steps of ultraviolet light irradiation which introduces single-strand nicks in ethidium bromide-stained DNA, converting CCC into OC forms. Unambiguous assignments of OC and CCC forms are possible in samples containing several plasmids.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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