Analysis of the complete nucleotide sequence of the<i>Agrobacterium tumefaciens vir</i>B operon
Nucleic Acids ResearchPublished 1 January 1988Open access
David V. Thompson, Leo S. Melchers, Ken B. Idler, R. A. Schilperoort, Paul J. J. Hooykaas
Citations101
SJR quartileQ1
SJR score7.78
SNIP4.71
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TL;DR
The complete nucleotide sequence of the virB locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined and both a membrane protein and a potential cytoplasmic protein contain the consensus amino acid sequence of ATP-binding proteins.
Abstract
The complete nucleotide sequence of the virB locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined. In the large virB-operon (9600 nucleotides) we have identified eleven open reading frames, designated virB1 to virB11. From DNA sequence analysis it is proposed that nearly all VirB products, i.e. VirB1 to VirB9, are secreted or membrane associated proteins. Interestingly, both a membrane protein (VirB4) and a potential cytoplasmic protein (VirB11) contain the consensus amino acid sequence of ATP-binding proteins. In view of the conjugative T-DNA transfer model, the VirB proteins are suggested to act at the bacterial surface and there play an important role in directing T-DNA transfer to plant cells.
Keywords
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