Regulation of the <i>Drosophila lin‐41</i> homologue <i>dappled</i> by <i>let‐7</i> reveals conservation of a regulatory mechanism within the LIN‐41 subclade
Developmental DynamicsPublished 10 December 2007Open access
Fergal O’Farrell, Shiva Seyedoleslami Esfahani, Ylva Engström, Per Kylsten
Citations37
SJR quartileQ2
SJR score0.86
SNIP0.76
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Abstract
Drosophila Dappled (DPLD) is a member of the RBCC/TRIM superfamily, a protein family involved in numerous diverse processes such as developmental timing and asymmetric cell divisions. DPLD belongs to the LIN-41 subclade, several members of which are micro RNA (miRNA) regulated. We re-examined the LIN-41 subclade members and their relation to other RBCC/TRIMs and dpld paralogs, and identified a new Drosophila muscle specific RBCC/TRIM: Another B-Box Affiliate, ABBA. In silico predictions of candidate miRNA regulators of dpld identified let-7 as the strongest candidate. Overexpression of dpld led to abnormal eye development, indicating that strict regulation of dpld mRNA levels is crucial for normal eye development. This phenotype was sensitive to let-7 dosage, suggesting let-7 regulation of dpld in the eye disc. A cell-based assay verified let-7 miRNA down-regulation of dpld expression by means of its 3'-untranslated region. Thus, dpld seems also to be miRNA regulated, suggesting that miRNAs represent an ancient mechanism of LIN-41 regulation.
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Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
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