Multiple promoters direct tissue-specific expression of the rat BDNF gene
NeuronPublished 1 March 1993
Tõnis Timmusk, Kaia Palm, Madis Metsis, Tõnu Reintam, Viiu Paalme, Märt Saarma
Citations859
SJR quartileQ1
SJR score6.75
SNIP2.95
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TL;DR
The data demonstrate that alternative usage of four promoters within the BDNF gene and differential splicing control tissue-specific and seizure-induced expression of BDNF mRNA.
Abstract
Brain-derived neurotrophic factor (BDNF) supports the survival of a specific set of neurons in the vertebrate nervous system. Here we show that the rat BDNF gene consists of four short 5' exons and one 3' exon encoding the mature BDNF protein. Eight different BDNF mRNAs with four different 5' ends and two alternative polyadenylation sites are transcribed from this gene. BDNF mRNAs containing exons I, II, and III are expressed predominantly in the brain, whereas exon IV transcripts predominate in the lung and heart. mRNAs containing exons I, II, and III increase markedly in the brain after kainic acid-induced seizures, whereas exon IV mRNA increases only slightly. Several transcription initiation sites were mapped upstream of the four 5' exons, and transfection of promoter-reporter gene constructs confirmed that these sequences act as promoters. Combined, the data demonstrate that alternative usage of four promoters within the BDNF gene and differential splicing control tissue-specific and seizure-induced expression of BDNF mRNA.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
Analytical BiochemistryA Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding
225,378 Citations1976Mark A. Bradford
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211,158 Citations1976Marion M. Bradford
BiochemistryIsolation of biologically active ribonucleic acid from sources enriched in ribonuclease
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A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
Molecular and Cellular BiologyRecombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
7,757 Citations1982C Gorman, Lindsey Moffat +1 more
Proceedings of the National Academy of SciencesPurification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
6,888 Citations1972Haim Aviv, Philip Leder
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.
Annual Review of BiochemistryOrganization and Expression of Eucaryotic Split Genes Coding for Proteins
4,641 Citations1981Richard Breathnach, Pierre Chambon
This paper organizes the organization of protein codes into split genes, a small number of which are expressed in the chickenuct, and discusses generalization, generalization and Molecular Evolution.
Nucleic Acids ResearchOptimal computer folding of large RNA sequences using thermodynamics and auxiliary information
3,628 Citations1981Michael Zuker, Patrick Stiegler
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Demonstration of selective degeneration of neurons of the nucleus basalis of Meynert represents the first documentation of a loss of a transmitter-specific neuronal population in a major disorder of higher cortical function and points to a critical subcortical lesion in Alzheimer's patients.
Journal of Molecular BiologyCpG Islands in vertebrate genomes
3,429 Citations1987Margaret Gardiner‐Garden, Marianne Frommer
It is shown that CpG islands in methylated genomes are maintained, despite a tendency for 5mCpG to mutate by deamination to TpG+CpA, by the structural stability of a high G+C content alone, and that C pG islands associated with exons result from some selective importance of the arginine codon CGX.
ScienceThe Nerve Growth Factor 35 Years Later
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The field of experimental embryology, which had been enthusiastically acclaimed in the mid-thirties, suffered from a sharp decrease in the enthusiasm that had inflamed the pioneers in this field, ever since R. G. Harrison delivered his celebrated lecture at the Royal Society in London in 1935.
The EMBO JournalPurification of a new neurotrophic factor from mammalian brain.
1,810 Citations1982Yves‐Alain Barde, David Edgar +1 more
This factor is the first neurotrophic factor to be purified since NGF, from which it is clearly distinguished because it has different antigenic and functional properties.
Nucleic Acids ResearchCAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements
1,758 Citations1987Bruno Luckow, Günther Schütz
In the promoterless construction pBLCAT3 eight unique restriction sites are suitable for insertion of different eukaryotic promoters at the 5' end of the CAT gene, enabling the excision of the intact fusion gene from the prokaryotic vector.
The Journal of Cell BiologyAn analysis of vertebrate mRNA sequences: intimations of translational control.
1,648 Citations1991M Kozak
CellThe “initiator” as a transcription control element
1,561 Citations1989Stephen T. Smale, David Baltimore
The Inr constitutes the simplest functional promoter that has been identified and provides one explanation for how promoters that lack TATA elements direct transcription initiation.
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NeuronBDNF mRNA expression is increased in adult rat forebrain after limbic seizures: Temporal patterns of induction distinct from NGF
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Proceedings of the National Academy of SciencesMolecular cloning and neurotrophic activities of a protein with structural similarities to nerve growth factor: developmental and topographical expression in the brain.
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533 Citations1990Heidi Phillips, Jeanne M. Hains +3 more
In human hippocampus, cells expressing BDNF mRNA are distributed in a fashion similar to that observed in the rat, which point to both basal forebrain cholinergic cells and olfactory pathways as potential central targets for BDNF.
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Proceedings of the National Academy of SciencesDifferential regulation of mRNAs for nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 in the adult rat brain following cerebral ischemia and hypoglycemic coma.
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GenomicsHuman and rat brain-derived neurotrophic factor and neurotrophin-3: Gene structures, distributions, and chromosomal localizations
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European Journal of NeuroscienceCells Expressing mRNA for Neurotrophins and their Receptors During Embryonic Rat Development
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In situ hybridization analysis of cells expressing messenger RNAs for the neurotrophins nerve growth factor, brain‐derived neurotrophic factor and neurotrophin‐3 and their high‐affinity receptors in the rat embryo revealed a complex but specific expression pattern for each of these mRNAs, suggesting a broad function in supporting neural innervation during embryonic development.
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The conclusion that seizure activity stimulates a transient increase in NGF expression by select populations of forebrain neurons is supported and indicates that experimental seizure paradigms might be further exploited for analyses of the mechanisms of NGF regulation and processing in the adult brain.
Journal of VirologyRegulation of cytomegalovirus gene expression: alpha and beta promoters are trans activated by viral functions in permissive human fibroblasts
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Annual Review of GeneticsALTERNATIVE PROMOTERS IN DEVELOPMENTAL GENE EXPRESSION
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In the dentate gyrus at postnatal day 13, intense BDNF RNA expression was limited to a defined area at the border between granule cell and molecular layers, suggesting the possibility that segregation of BDNF mRNA into defined subcellular compartments may play a role in establishing the well-delineated patterns of innervation in the hippocampus.
European Journal of NeuroscienceCells that Express Brain‐Derived Neurotrophic Factor mRNA in the Developing Postnatal Rat Brain
207 Citations1991Wilma Friedman, Lars Olson +1 more
Both transient and persistent expression of BDNF mRNA in various regions of the developing rat brain are shown and suggest that there is a caudal to rostral gradient ofBDNF expression during postnatal brain development, which may be correlated to neuronal maturation.
FEBS LettersCloning and expression of a cDNA encoding a novel human neurotrophic factor
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The polypyrimidine tract, so distinctive of vertebrate introns, is not a trait of the introns in the non-vertebrate organisms examined in this study and thus in some aspects may be atypical.
Molecular and Cellular BiologyMouse nerve growth factor gene: structure and expression.
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124 Citations1991Linda H. Shapiro, RA Ashmun +2 more
Both aminopeptidase N transcripts encode the same polypeptide, indicating that the physically distinct promoters must have evolved to regulate expression of this cell-surface peptidase by cells of different tissues.
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A combined mechanism involving the use of tissue-specific alternative promoters and the differential splicing of exon 1 generates the mature GHRH transcript in placenta and hypothalamus, suggesting that the mature placental G HRH is identical to its hypothalamic counterpart.
Journal of Biological ChemistryDifferential association of insulin-like growth factor I mRNA variants with polysomes in vivo.
72 Citations1991Howard L. Foyt, Derek LeRoith +1 more
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The results indicate that transcriptional activator as well as suppressor regions surround the NGF gene promoter, and the regulation of NGF production is likely to involve cis elements within these regions and transacting factors that bind to them.
Nucleic Acids ResearchUpstream box/TATA box order is the major determinant of the direction of transcription
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