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Salt induced transitions of chromatin core particles studied by tyrosine fluorescence anisotropy

Nucleic Acids ResearchPublished 1 January 1980Open access
Louis J. Libertini, Enoch W. Small
Citations90
SJR quartileQ1
SJR score7.78
SNIP4.71
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TL;DR

Chromatin core particles containing 146 base pairs of DNA have been found to undergo a single defined transition below 10 mM ionic strength as studied by both sedimentation velocity and tyrosine fluorescence anisotropy.

Abstract

Chromatin core particles containing 146 base pairs of DNA have been found to undergo a single defined transition below 10 mM ionic strength as studied by both sedimentation velocity and tyrosine fluorescence anisotropy. A method is described for the preparation of such core particles from chicken erythrocytes with greater than 50% yield.

Keywords

Biochemistry, Genetics and Molecular Biology