Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids.
PubMedPublished 1 June 1986
Margaret L. Van Keuren, Paul C. Watkins, HA Drabkin, Ethylin Wang Jabs, James F. Gusella, David Patterson
Citations83
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TL;DR
A panel of Chinese hamster X human somatic cell hybrids, each containing various portions of chromosome 21 as the only detectable human chromosome component, is used for regional mapping of cloned, chromosome 21-derived DNA sequences.
Abstract
We have used a panel of Chinese hamster X human somatic cell hybrids, each containing various portions of chromosome 21 as the only detectable human chromosome component, for regional mapping of cloned, chromosome 21-derived DNA sequences. Thirty unique and very low-repeat sequences were mapped to the short arm and three sections of the long arm. Three unique sequences map to the proximal part of the terminal band 21q22.3, and five to the distal part of this band. Some of these may represent parts of gene sequences that may be relevant to the pathogenesis of Down syndrome, as 21q22 is the area required to be present in triplicate for the full clinical picture.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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