Modification of proteins with covalent lipids
Progress in Lipid ResearchPublished 1 January 1988Open access
Eric N. Olson
Citations50
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MedicineBiochemistry, Genetics and Molecular Biology
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175 Citations1986Janice E. Buss, Mark P. Kamps +2 more
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Molecular and Cellular BiologyMyristic acid is attached to the transforming protein of Rous sarcoma virus during or immediately after synthesis and is present in both soluble and membrane-bound forms of the protein.
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171 Citations1987David H. Persing, Harold Varmus +1 more
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163 Citations1987Akira Ishihara, Y. Hou +1 more
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Journal of Biological ChemistryAcylation of disc membrane rhodopsin may be nonenzymatic.
158 Citations1987Peter J. O’Brien, Robert St. Jules +3 more
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154 Citations1986Janice E. Buss, Bartholomew M. Sefton
Molecular and Cellular BiologyA Short Sequence in the p60<sup><i>src</i></sup> N Terminus Is Required for p60<sup><i>src</i></sup> Myristylation and Membrane Association and for Cell Transformation
149 Citations1984Frederick R. Cross, Ellen A. Garber +2 more
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The Journal of Cell BiologyDissection of the Golgi complex. II. Density separation of specific Golgi functions in virally infected cells treated with monensin.
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Journal of Biological ChemistryIdentification of the NH2-terminal blocking group of NADH-cytochrome b5 reductase as myristic acid and the complete amino acid sequence of the membrane-binding domain.
145 Citations1984Juris Ozols, Steven A. Carr +1 more
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The Journal of Cell BiologyPosttranslational modification and intracellular transport of a trypanosome variant surface glycoprotein.
144 Citations1986James D. Bangs, Norma W. Andrews +2 more
Journal of Biological ChemistryAcylation of a Plasmodium falciparum merozoite surface antigen via sn-1,2-diacyl glycerol.
142 Citations1985Kasturi Haldar, Michael A. J. Ferguson +1 more
Enzymatic treatment of the purified acylated protein established that the lipid is present as sn-1,2-diacyl glycerol, most probably linked to a phosphodiester at the 3-position of glycerl.
Biochemical and Biophysical Research CommunicationsOn the type of linkage binding fatty acids present in brain white matter proteolipid apoprotein
139 Citations1971Pierre Stoffyn, J. Folch‐Pi
Fatty acids (FA) which constitute about 2.5% by weight of exhaustively delipidated proteolipid apoprotein (PLA) have been shown to be bound mainly, if not exclusively, by ester linkages.
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136 Citations1985Nancy R. Rice, Robert M. Stephens +2 more
The BLV gag and pol gene products are highly related to those of the human T-cell leukemia virus (HTLV); relatedness varied from 37% amino acid identities within the N terminal gag protein to 54% within the nucleic acid binding protein.
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135 Citations1985Anthony H. Futerman, Rosamaria Fiorini +3 more
The results presented suggest that DSAChE is anchored to the plasma membrane by one or more PI molecules which are tightly attached to a short amino acid sequence at one end of the catalytic subunit polypeptide.
Current topics in microbiology and immunologyFatty Acid Binding: A New Kind of Posttranslational Modification of Membrane Proteins
133 Citations1983Michael F.G. Schmidt
Many cellular functions are carried out by proteins which are in close association with lipid bilayers which are of utmost importance for receiving extracellular signals as, for instance, through the binding of antigens, hormones, neurotransmitters, lectins, antibodies, neighboring cells, or viruses.
Journal of Biological ChemistrySubstrate recognition by oligosaccharyltransferase. Studies on glycosylation of modified Asn-X-Thr/Ser tripeptides.
132 Citations1983J K Welply, P Shenbagamurthi +2 more
NH2-terminal derivatives of Asn-Leu-Thr-NH2 and peptides with asparagine replacements have been tested as substrates or inhibitors of N-glycosylation and suggest that the tripeptides are freely permeable to the microsomal membrane.
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128 Citations1982Anthony I. Magee, Milton J. Schlesinger
Journal of Biological ChemistryCerulenin blocks fatty acid acylation of glycoproteins and inhibits vesicular stomatitis and Sindbis virus particle formation.
128 Citations1982Milton J. Schlesinger, C Malfer
Data indicate that fatty acid acylation is not essential for intracellular transport of these membrane proteins, but it may have an important role in the interaction of glycoproteins with membranes during virus assembly and budding.
Hoppe-Seyler´s Zeitschrift für physiologische ChemieThe Primary Structure of Bovine Brain Myelin Lipophilin (Proteolipid Apoprotein)
126 Citations1983Wilhelm Stoffel, Heinz Hillen +2 more
On the basis of structural data a model for the membrane integration of lipophilin is proposed and the covalent fatty acyl bond is being discussed together with important structural and functional properties of this membrane protein which can be derived from sequence information.
Proceedings of the National Academy of SciencesProtein fatty acid acylation: enzymatic synthesis of an N-myristoylglycyl peptide.
124 Citations1986Dwight A. Towler, L Glaser
An enzymatic activity in yeast is described that will transfer myristic acid to the amino terminus of the octapeptide Gly-Asn-Ala- ala-AlA-Alas-Als-Arg-Arg, whose sequence was derived from a known N-myristoylated acyl protein, the catalytic subunit of cAMP-dependent protein kinase of bovine cardiac muscle.
The Journal of Cell BiologyHigh lateral mobility of endogenous and transfected alkaline phosphatase: a phosphatidylinositol-anchored membrane protein.
123 Citations1987Makoto Noda, Kyung Chul Yoon +2 more
Results indicate that the transfected cells used in this study possess the machinery for AP insertion into the membrane and its binding to PI, a conclusion with general implications for the understanding of the slow diffusion of other membrane proteins.
Journal of Biological ChemistryPrevention of NH2-terminal acetylation of proteins synthesized in cell-free systems.
121 Citations1977R D Palmiter
The NH2 terminus of ovalbumin is acetylated in cell-free protein-synthesizing systems as it is in vivo and can be sequenced by automated Edman degradation.
Proceedings of the National Academy of SciencesCovalently bound myristate in a lymphoma tyrosine protein kinase.
120 Citations1984G.A. Marchildon, J E Casnellie +2 more
The murine lymphoma cell line LSTRA expresses high levels of a membrane-associated tyrosine protein kinase, which is now show to be acylated by [3H]myristate in vivo, and chemical characterization of the immunoprecipitated protein isolated by NaDodSO4/PAGE verifies that the 3H label is in protein-associated myristate.
Journal of VirologyPosttranslational processing of p21 ras proteins involves palmitylation of the C-terminal tetrapeptide containing cysteine-186
120 Citations1985Z Q Chen, Linda S. Ulsh +2 more
It is suggested that the enzymatic mechanism for p21 palmitylation may be different from N-terminal myristylation of many other membrane proteins.
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118 Citations1981Heiner Niemann, Hans‐Dieter Klenk
Coronaviruses are a suitable model system to study biosynthesis and processing of this type of glycoprotein and E1 is the first viral glycop protein analyzed that contains only 0-glycosidic linkages.
Journal of Biological ChemistryFatty acylation of cellular proteins. Temporal and subcellular differences between palmitate and myristate acylation.
118 Citations1986Eric N. Olson, Gwendolyn Spizz
The kinetics and subcellular sites of acylation of proteins with palmitate and myristate were examined and the lack of carbohydrate on the majority of integral membrane acyl proteins suggests that these proteins may follow intracellular pathways that are different from those followed by cell surface glycoproteins.
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111 Citations1986Hans Reiser, Hans C. Oettgen +5 more
The T-cell-activating protein (TAP), an Ly-6 gene product involved in T cell activation, is characterized as a glycoprotein with a molecular weight of 10-12 kd under nonreducing conditions and 15-18 kD under reducing ones, and appears to be attached to the cell membrane via this lipid.
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111 Citations1984Anna Voronova, J E Buss +3 more
Proceedings of the National Academy of SciencesCapsid protein VP4 of poliovirus is N-myristoylated.
108 Citations1987Aniko V. Paul, A M Schultz +3 more
Evidence is presented showing unambiguously that the N-terminal glycine residue of VP4 is N-myristoylated, and the possible function of the myristic acid residue in VP4 and in its precursor in poliovirus proliferation is discussed.
Journal of BacteriologyCharacterization of the Phospholipases of<i>Bacillus cereus</i>and Their Effects on Erythrocytes, Bone, and Kidney Cells
108 Citations1965Milton W. Slein, Gerald F. Logan
A sensitive kidney cortex homogenate test is described that depends on the release of alkaline phosphatase for the measurement of phosphatasemia factor (PF) activity associated with the phospholipases.
NatureMyristic acid is coupled to a structural protein of polyoma virus and SV40
106 Citations1987Charles Streuli, Beverly E. Griffin
It is shown that VP2 from both polyoma virus and simian virus 40 (SV40) is covalently linked to myristic acid; this is the first report of a myristylated protein in the nucleus and of a fatty acid being important in the structure of a non-enveloped virus.
BiochemistryIdentification of amine components in a glycolipid membrane-binding domain at the C-terminus of human erythrocyte acetylcholinesterase
105 Citations1986Robert Haas, Patricia T. Brandt +2 more
The membrane-binding domain of human erythrocyte acetylcholinesterase is a covalently linked glycolipid at the C-termini of the subunits that is susceptible to radiomethylation in the intact enzyme.
Journal of Biological ChemistryAlpha and beta subunits of the nicotinic acetylcholine receptor contain covalently bound lipid.
105 Citations1984Eric N. Olson, L Glaser +1 more
Treatment of differentiated BC3H1 cells with cerulenin resulted in a 50% inhibition in expression of the acetylcholine receptor on the cell surface under conditions where there was minimal inhibition of protein synthesis.
Biochemical JournalCarbohydrate is linked through ethanolamine to the <i>C</i>-terminal amino acid of <i>Trypanosoma brucei</i> variant surface glycoprotein
102 Citations1983Anthony A. Holder
The C-terminal amino acid of the variant surface glycoprotein from Trypanosoma brucei is glycosylated and it was shown that the sugar side chain is linked through ethanolamine to the alpha-carboxy group of the amino acid.
The EMBO JournalStudies on the attachment of myristic and palmitic acid to cell proteins in human squamous carcinoma cell lines: evidence for two pathways.
100 Citations1985R. A. Jeffrey McIlhinney, S.J. Pelly +2 more
Observations show for the first time that two different protein populations are labelled by different lipids in eukaryotic cells, and that there appear to be two separate pathways for the acylation of proteins in such cells.
Proceedings of the National Academy of SciencesIdentification of the parasite transferrin receptor of Plasmodium falciparum-infected erythrocytes and its acylation via 1,2-diacyl-sn-glycerol.
97 Citations1986Kasturi Haldar, C L Henderson +1 more
Preliminary analysis indicated that, unlike the human receptor, the plasmodial transferrin receptor is not a disulfide linked dimer but a single polypeptide acylated via 1,2-diacyl-sn-glycerol.
Proceedings of the National Academy of SciencesAnkyrin is fatty acid acylated in erythrocytes.
91 Citations1986Matthias Staufenbiel, Elias Lazarides
It is shown that [3H]palmitic acid is incorporated into ankyrin in vivo, suggesting that the fatty acid bound to ankyr in turns over more rapidly than the polypeptide.
Molecular and Cellular BiologyInteraction between the Rous sarcoma virus transforming protein and two cellular phosphoproteins: analysis of the turnover and distribution of this complex.
89 Citations1983Joan S. Brugge, W Yonemoto +1 more
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