10Sa RNA, a small stable RNA of Escherichia coli, is functional
Molecular and General Genetics MGGPublished 1 September 1991
Bong-Kyeong Oh, David Apirion
Citations83
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A mutant in which the chromosomal 10 Sa RNA gene is interrupted by a cat insert does not produce detectable levels of 10Sa RNA, and it grows more slowly than the parental strain.
Abstract
The ssrA gene, coding for the metabolically stable 10Sa RNA, affects cell growth. A mutant in which the chromosomal 10Sa RNA gene is interrupted by a cat insert does not produce detectable levels of 10Sa RNA, and it grows more slowly than the parental strain.
Keywords
Biochemistry, Genetics and Molecular Biology
Molecular Cloning: A Laboratory Manual 2
129,982 Citations1989Joseph Sambrook, Elisabeth Fritsch +1 more
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
Journal of Molecular BiologyDetection of specific sequences among DNA fragments separated by gel electrophoresis
32,820 Citations1975E.M. Southern
This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Analytical BiochemistryA technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
25,157 Citations1983Andrew P. Feinberg, Bert Vogelstein
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
Nucleic Acids ResearchA rapid alkaline extraction procedure for screening recombinant plasmid DNA
14,877 Citations1979H.C. Birnboim, Janine Doly
The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Proceedings of the National Academy of SciencesHybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
8,386 Citations1980Patricia S. Thomas
A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Molecular and Cellular BiologyRecombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
7,757 Citations1982C Gorman, Lindsey Moffat +1 more
Molecular and Cellular BiologyRecombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells
3,106 Citations1982Cornelia M. Gorman, Leslie F. Moffat +1 more
A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
Nucleic Acids ResearchRapid and efficient cosmid cloning
1,821 Citations1981David Ish‐Horowicz, J. F. Burke
The method eliminates the need to size the insert DNA fragments and prevents formation of clones containing short or multiple inserts and a rapid and efficient method for preparing plasmid and cosmid DNA is described.
CellThe physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library
1,763 Citations1987Yuji Kohara, Kiyotaka Akiyama +1 more
The strategy was to measure the sizes of partial restriction enzyme digests by hybridization with a vector probe in a manner analogous to nucleotide sequencing, and found that this strategy is applicable to analyses of the genomes of other organisms.
Journal of BacteriologyNew method for generating deletions and gene replacements in Escherichia coli
686 Citations1989Carol M. Hamilton, Martí Aldea +3 more
A method for generating gene replacements and deletions in Escherichia coli using a temperature-sensitive pSC101 replicon to facilitate the gene replacement and can be used to generate deletions of essential genes.
Journal of BacteriologySite-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli
339 Citations1985Stephen C. Winans, Stephen J. Elledge +2 more
A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant.
GeneConstruction and characterization of the chloramphenicol-resistance gene cartridge: A new approach to the transcriptional mapping of extrachromosomal elements
303 Citations1982Timothy J. Close, Raymond L. Rodriguez
A system based upon the activity of the Tn 9 Cm r gene whose product, chloramphenicol acetyltransferase (CAT), can be easily assayed enzymatically and identified phenotypically in Escherichia coli and it should be possible to study gene expression on any extrachromosomal element in the appropriate host.
Journal of BacteriologyDeletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments
148 Citations1984Maria Jasin, Paul Schimmel
Deletion of an essential gene in Escherichia coli was accomplished by transformation of linear DNA fragments that have a Kanr gene segment flanked by sequences homologous to closely spaced regions on the E. coli chromosome.
Journal of Biological ChemistryMultiple pathways for primary processing of ribosomal RNA in Escherichia coli.
141 Citations1977Peter Gegenheimer, Ned Watson +1 more
It is suggested that in the absence of RNase III, which is involved in the primary processing of rRNA in E. coli, other enzymes involved in primary and secondaryprocessing of r RNA in RNaseIII+ cells can recognize their sites on the nascent rRNA transcript and accomplish the primaryprocessing.
Microbiological ReviewsProcessing of procaryotic ribonucleic acid
135 Citations1981Peter Gegenheimer, David Apirion
This paper discusses RNA Processing Mechanisms, which consists of Polycistronic Transcription and Processing, and its applications in Transcript Processing and Attenuation, and Mutants Defective in RNA Processing.
Journal of BacteriologySmall Stable RNAs from <i>Escherichia coli</i> : Evidence for the Existence of New Molecules and for a New Ribonucleoprotein Particle Containing 6S RNA
117 Citations1978Suzanne Y. Lee, Stephen C. Bailey +1 more
Small stable RNA molecules of Escherichia coli other than 5S (rRNA) and 4S (tRNA) were studied and 6S RNA was unique in that it seemed to exist in a ribonucleoprotein particle.
Journal of Biological ChemistryExpression and characterization of RNase III and Era proteins. Products of the rnc operon of Escherichia coli.
94 Citations1990S M Chen, Howard Takiff +4 more
Plasmids that overproduce RNase III and/or Era proteins under the control of the lambda PL promoter are constructed and purified Era protein binds GDP and GTP and has GTPase activity.
Proceedings of the National Academy of SciencesFunctional expression in yeast of the Escherichia coli plasmid gene coding for chloramphenicol acetyltransferase.
87 Citations1980Joe D. Cohen, T. Ross Eccleshall +4 more
The Escherichia coli R factor-derived chloramphenicol resistance (camr) gene is functionally expressed in the yeast Saccharomyces cerevisiae and segregate with the yeast marker LEU2, carried by the transforming plasmid, during both mitotic growth and meiotic division.
Molecular and General Genetics MGGCharacterization of 10S RNA: A new stable RNA molecule from Escherichia coli
84 Citations1979Bimal K. Ray, David Apirion
10S RNA cannot be processed by cell extracts to tRNA-sized molecules and does not bind significantly to ribosomes, hence it is unlikely to be a tRNA precursor or an mRNA.
Methods in enzymology on CD-ROM/Methods in enzymology[68] Preparation of bacterial DNA by the phenol-pH 9-RNases method
78 Citations1967Kin‐ichiro Miura
This chapter describes the method for the extraction of DNA from bacterial cells using phenol, which is considered one of the most effective methods for deproteinization.
Molecular MicrobiologyThe gene for a small stable RNA (10Sa RNA) of <i>Escherichia coli</i>
70 Citations1989Anil K. Chauhan, David Apirion
A gene that codes for a small stable RNA (362 nucleotides) has been sequenced and it is a monocistronic gene, with its own promoter and terminator.
Journal of Molecular BiologyA small RNA that complements mutants in the RNA processing enzyme ribonuclease P
60 Citations1982Swatantra Kumar Jain, Michael Gurevitz +1 more
Structural analysis revealed that the 10 S a and 10 S b RNA molecules have dissimilar sequences, which are unique among all RNAs tested thus far, and they become rather labile when RNA synthesis is blocked by rifampicin.
Molecular and General Genetics MGGA precursor for a small stable RNA (10Sa RNA) ofEscherichia coli
41 Citations1989Makam N. Subbarao, David Apirion
Strains carrying plasmids that code for 10Sa RNA synthesize a larger molecule when the RNA processing enzyme RNase E is inactivated, and it is shown that the larger RNA is converted to the smaller, mature RNA.
Nucleic Acids ResearchPrecursors to 16S and 23S ribosomal RNA from a ribonuclease III<sup>−</sup>strain of Escherichia coli contain intact RNase III processing sites
39 Citations1980Peter Gegenheimer, David Apirion
The experiments demonstrate the absence of significant RNase III processing activity in rnc-105 strains of E. coli, and implicate the participation of another endonuclease(s) in rRNA processing in mutant and wild-type cells.
Nucleic Acids ResearchNucleotide sequence of the 10Sa RNA gene of the βpurple eubacterium<i>Alcaligenes eutrophus</i>
33 Citations1990James W. Brown, Dirk A Hunt +1 more
The cloned DNA hybridizes to a 340 nucleotide RNA in Northern blots of total RNA from A. eutrophus and another ,B-purple eubacterium, Pseudomonas testosteroni, but not other organisms tested, including E. coli (data not shown).
Journal of BacteriologyLocation of a gene (ssrA) for a small, stable RNA (10Sa RNA) in the Escherichia coli chromosome
29 Citations1990Bong-Kyeong Oh, Anil K. Chauhan +2 more
The gene for 10Sa RNA, which is a major small, stable RNA in Escherichia coli, is a unique gene in the E. coli chromosome.
Molecular and General Genetics MGGConsequences of losing ribonuclease III on theEscherichia coli cell
29 Citations1976David Apirion, Jeff Neil +1 more
Comparing the decay of newly synthesized RNA and functional decay of β-galactosidase mRNA inEscherichia coli strains revealed that in both strains these parameters proceed in similar rates, which suggests that RNase III is not involved in the metabolism of mRNA.
Molecular and General Genetics MGGRevertants from RNase III negative strains of Escherichia coli
15 Citations1976David Apirion, Jeff Neil +1 more
All the effects observed inRNase III- strains are due to pleiotropic effects of the rnc-105 allele, and that the enzyme RNase III is not essential for the viability of the E. coli cell.