Partial purification and properties of an endonuclease from germinating pea seeds specific for single-stranded DNA
Archives of Biochemistry and BiophysicsPublished 1 August 1979
Altaf A. Wani, S.M. Hadi
Citations23
SJR quartileQ1
SJR score0.91
SNIP0.78
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TL;DR
An enzyme that rapidly catalyzes the hydrolysis of denatured DNA has been partially purified from germinated pea seeds and shows activity over a broad range of pH but was most active between pH 6.5 and 8.0.
Abstract
An enzyme that rapidly catalyzes the hydrolysis of denatured DNA has been partially purified from germinated pea (Pisum sativum) seeds. The nuclease has been characterised as having endonucleolytic activity degrading single stranded DNA at a 15- to 20-fold higher rate than native DNA. From exclusion chromatography on Sephadex G-200 the molecular weight of the enzyme was calculated to be 42,000. The small extent of hydrolysis of native DNA is suggested to be due to the degradation of partially denatured areas in the native molecule. The enzyme shows activity over a broad range of pH but was most active between pH 6.5 and 8.0. The maximum hydrolysis of denatured DNA was observed at 45 °C while with native DNA the temperature optima was 60 °C. The nuclease does not show an absolute requirement for added divalent cations. However, the addition of Mg2+ and Ca2+ results in 40 and 60% stimulation, respectively. EDTA has no effect on enzymatic activity, whereas 8-hydroxyquinoline was inhibitory.
Keywords
Biochemistry, Genetics and Molecular Biology
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