Foamy virus vectors
Journal of VirologyPublished 1 January 1996Open access
David W. Russell, A. Dusty Miller
Citations174
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SJR score1.28
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TL;DR
V vectors based on human foamy virus that encode neomycin phosphotransferase and alkaline phosphatase are constructed and are able to transduce a wide variety of vertebrate cells by integration of the vector genome.
Abstract
Human foamy virus (HFV) is a retrovirus of the spumavirus family. We have constructed vectors based on HFV that encode neomycin phosphotransferase and alkaline phosphatase. These vectors are able to transduce a wide variety of vertebrate cells by integration of the vector genome. Unlike vectors based on murine leukemia virus, HFV vectors are not inactivated by human serum, and they transduce stationary-phase cultures more efficiently than murine leukemia virus vectors. These properties, as well as their large packaging capacity, make HFV vectors promising gene transfer vehicles.
Keywords
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PubMedImproved retroviral vectors for gene transfer and expression.
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Journal of BacteriologyPersistent Infection of a Rat Kidney Cell Line with Rauscher Murine Leukemia Virus
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Molecular and Cellular BiologyGene Transfer by Retrovirus Vectors Occurs Only in Cells That Are Actively Replicating at the Time of Infection
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JNCI Journal of the National Cancer InstituteAn Unusual Virus in Cultures From a Human Nasopharyngeal Carcinoma<xref ref-type="fn" rid="FN2">2</xref>
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