Techniques in Clinical Immunology
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Abstract
There are clinical immunology laboratories across the world that not only provide numerical and qualitative data, but also expert clinical understanding for physicians and paediatricians for whom there is little training in laboratory techniques or interpretation. Serum immunoglobulin measurements are essential in patients with repeated or severe infections and those with myeloma and some other lymphoproliferative disorders. Assays for complement components in the serum are divided into those assays that recognize the antigenic nature of the individual complement components and those that measure functional activity, such as cell lysis. Detection of antimicrobial antibodies is an essential part of the investigation of immune deficiency. The detection of circulating autoantibodies commonly involves one of five methods: immunofluorescence, radioimmunoassay, enzyme-linked immunoassay, countercurrent electrophoresis and cell-based flow cytometry. Tests of neutrophil function are essential in patients with recurrent or severe staphylococcal or fungal infections.