A Genome-Wide Map of Conserved MicroRNA Targets in C. elegans
Current BiologyPublished 4 February 2006Open access
Sabbi Lall, Dominic Grün, Azra Krek, Kevin Chen, Yi-Lu Wang, Colin N. Dewey
Citations421
SJR quartileQ1
SJR score2.71
SNIP1.83
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TL;DR
A new experimental pipeline to assay 3' UTR-mediated posttranscriptional gene regulation via an endogenous reporter expression system amenable to high-throughput cloning is developed, demonstrating the utility of this system using one of the most intensely studied miRNAs, let-7.
Abstract
At least 10% of C. elegans genes are predicted miRNA targets, and a number of nematode miRNAs seem to regulate biological processes by targeting functionally related genes. We have also developed and successfully utilized an in vivo system for testing miRNA target predictions in likely endogenous expression domains. The thousands of genome-wide miRNA target predictions for nematodes, humans, and flies are available from the PicTar website and are linked to an accessible graphical network-browsing tool allowing exploration of miRNA target predictions in the context of various functional genomic data resources.
Keywords
Biochemistry, Genetics and Molecular Biology
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