Fragments of the simian virus 40 transforming gene facilitate transformation of rat embryo cells.
Proceedings of the National Academy of SciencesPublished 1 September 1982Open access
Wendy W. Colby, Thomas Shenk
Citations129
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TL;DR
Segments of the simian virus 40 (SV40) genome that encode only fragments of large tumor antigen can facilitate immortalization of secondary rat embryo cells and does not appear to be essential for either immortalization or transformation.
Abstract
Segments of the simian virus 40 (SV40) genome that encode only fragments of large tumor antigen can facilitate immortalization of secondary rat embryo cells. The phenotypes of the immortalized cells range from nearly "normal" to fully transformed. All of the cell lines contain SV40 sequences and express unstable NH2-terminal fragments of large tumor antigen. SV40 small tumor antigen does not appear to be essential for either immortalization or transformation.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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