Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing<i>Escherichia coli</i>Strains from Calves
Journal of Clinical MicrobiologyPublished 1 June 1998Open access
Sophia M. Franck, Brad T. Bosworth, Harley W. Moon
Citations201
SJR quartileQ1
SJR score1.88
SNIP1.68
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TL;DR
A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, andShiga toxins 1 and 2.
Abstract
A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.
Keywords
Agricultural and Biological SciencesMedicineBiochemistry, Genetics and Molecular Biology
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The ability of enteropathogenic Escherichia coli to form attaching and effacing intestinal lesions is a major characteristic of EPEC pathogenesis and a chromosomal gene (eae) that is necessary for this activity is identified using TnphoA mutagenesis.
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