Regulation of cytochrome P1-450 gene transcription by 2,3,7, 8-tetrachlorodibenzo-p-dioxin in wild type and variant mouse hepatoma cells.
Journal of Biological ChemistryPublished 1 May 1984Open access
David I. Israel, James P. Whitlock
Citations166
SJR quartileQ1
SJR score1.71
SNIP1.00
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
It is demonstrated that TCDD increases the rate of transcription of the cytochrome P1-450 gene and suggested that transcription requires nuclear localization of the T CDD-receptor complex.
Abstract
Using RNA synthesized in isolated nuclei, we have measured the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the rate of synthesis of cytochrome P1-450 mRNA in wild type mouse hepatoma (Hepa 1c1c7) cells and in variant (BPrc1) cells, which fail to accumulate the TCDD-receptor complex within the nucleus. In wild type cells, TCDD induces a 20-fold increase in the rate of synthesis of cytochrome P1-450 mRNA within 30 min. This effect persists for at least 18 h. In contrast, TCDD has no effect on cytochrome P1-450 mRNA synthesis in the variant cells. The results demonstrate that TCDD increases the rate of transcription of the cytochrome P1-450 gene and suggest that transcription requires nuclear localization of the TCDD-receptor complex.
Keywords
Biochemistry, Genetics and Molecular BiologyPharmacology, Toxicology and PharmaceuticsEnvironmental Science
Journal of Molecular BiologyDetection of specific sequences among DNA fragments separated by gel electrophoresis
32,820 Citations1975E.M. Southern
This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
The Annual Review of Pharmacology and Toxicology2,3,7,8-Tetrachlorodibenzo-<i>p</i>-Dioxin and Related Halogenated Aromatic Hydrocarbons: Examination of the Mechanism of Toxicity
2,506 Citations1982Alan Poland, Joyce C. Knutson
The toxicity of halogenated aromatic hydrocarbons appears to be due to the sustained expression of a normal cellular regulatory system, of which the author was previously unaware.
Molecular and Cellular BiologyIsolation and Characterization of Full-Length cDNA Clones for Human α-, β-, and γ-Actin mRNAs: Skeletal but Not Cytoplasmic Actins Have an Amino-Terminal Cysteine that Is Subsequently Removed
1,211 Citations1983Peter W. Gunning, Phyllis Ponte +4 more
Analysis of the DNA sequences of the 5' end of the clones demonstrated that although alpha- and gamma-actin genes start with a methionine codon, it is likely that post-translational removal of cysteine in addition to methionines accompanies alpha-actIn synthesis but not beta- and Gamma-Actin synthesis.
Journal of Biological ChemistryTranscriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
971 Citations1979G. Stanley McKnight, Richard D. Palmiter
Relative rates of ovalbumin and conalbumin mRNA transcription were measured in isolated oviduct nuclei by allowing endogenous RNA polymerases to synthesize [32P]RNA that was then hybridized to immobilized recombinant DNA containing the respective gene sequences.
NatureVariety in the level of gene control in eukaryotic cells
772 Citations1982James Darnell
Definition of eukaryotic transcription units as simple and complex affords a framework in which to discuss control at the level of RNA processing for which several examples also are known.
Journal of Biological ChemistryGene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.
483 Citations1979Geoffrey M. Wahl, Richard A. Padgett +1 more
Restriction patterns were analyzed by a new method in which fragments of DNA are transferred from agarose gels to diazo paper with a high efficiency which is independent of size, and indicated that part of this difference is attributable to intervening sequences in the CAD gene.
Annual Review of BiochemistryTHE PATHWAY OF EUKARYOTIC mRNA FORMATION
431 Citations1983Joseph R. Nevins
This paper presents a monograph on the construction of a Transcriptional Unit and some of the properties of this unit, as well as some examples of use cases and applications.
The Annual Review of Pharmacology and ToxicologyGenetic Mechanisms Controlling the Induction of Polysubstrate Monooxygenase (P-450) Activities
295 Citations1981D W Nebert, Howard J. Eisen +4 more
Journal of Biological ChemistryTranscriptional regulation of rat liver epoxide hydratase, NADPH-Cytochrome P-450 oxidoreductase, and cytochrome P-450b genes by phenobarbital.
221 Citations1983James P. Hardwick, Frank J. Gonzalez +1 more
The rapid increase in the rates of transcription and the appearance of elevated levels of nuclear pre-mRNAs and cytoplasmic mRNAs unequivocally demonstrates that phenobarbital elevates levels of these drug-metabolizing enzymes primarily by augmenting the rate of transcription of their respective genes.
Journal of Biological ChemistryGlucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells.
210 Citations1981Kelly E. Mayo, Richard D. Palmiter
Induction of metallothionein-I mRNA appears to be independent of dexamethasone effects on zinc uptake and does not require concomitant protein synthesis.
NatureEpidermal growth factor rapidly stimulates prolactin gene transcription
201 Citations1982Geoffrey Murdoch, E Potter +3 more
It is demonstrated that the increased Prolactin synthesis appears to be the result of a rapid stimulation of prolactin gene transcription by EGF, as well as a partial inhibition of cell growth.
Journal of Biological ChemistryBiochemical and genetic analysis of variant mouse hepatoma cells defective in the induction of benzo(a)pyrene-metabolizing enzyme activity.
199 Citations1983A. Dusty Miller, David I. Israel +1 more
Cell fusions indicate that both variant phenotypes are recessive with respect to wild type, and implementation analyses indicate that the defects are located on different genes.
Biochemical PharmacologyMultiple forms of cytochrome P-450 and the importance of molecular biology and evolution
145 Citations1982Daniel W. Nebert, Masahiko Negishi
Journal of Biological Chemistry2,3,7,8-Tetrachlorodibenzo-p-dioxin receptors in wild type and variant mouse hepatoma cells. Nuclear location and strength of nuclear binding.
121 Citations1984James P. Whitlock, Donna R. Galeazzi
It is concluded that, in the intact cell, TCDD receptors are located within the nucleus and that the temperature-dependent event in the induction of cytochrome P1-450 gene expression is one which strengthens the binding of the T CDD-receptor complex to chromatin.
Journal of Biological ChemistryInduction of mRNA specific for cytochrome P1-450 in wild type and variant mouse hepatoma cells.
115 Citations1983David I. Israel, James P. Whitlock
It is concluded that expression of the cytochrome P1-450 gene is under transcriptional control and requires nuclear localization of the TCDD receptor.
Molecular Pharmacology1,4-Bis[2-(3,5-Dichloropyridyloxy)]Benzene, a Potent Phenobarbital-Like Inducer of Microsomal Monooxygenase Activity
107 Citations1980Alan Poland, I. Tong Mak +4 more
Despite the lack of a discernable structure-activity relationship among the numerous foreign compounds which evoke the phenobarbital pleiotropic response, the existence of a potent agonist, i.e., TCPOBOP, suggests that this response may be mediated through a specific recognition site.
Journal of Biological ChemistryRegion-specific initiation of mouse mammary tumor virus RNA synthesis by endogenous RNA polymerase II in preparations of cell nuclei.
97 Citations1983Michael R. Stallcup, L D Washington
The sensitivity of MMTV RNA initiation and synthesis to alpha-amanitin and actinomycin D are characteristic of DNA-directed transcription by RNA polymerase II.
Molecular PharmacologySpecies Differences in Responsiveness to 1,4-Bis[2-(3,5-dichloropyridyloxy)]-benzene, a Potent Phenobarbital-Like Inducer of Microsomal Monooxygenase Activity
72 Citations1981Alan Poland, I. Tong Mak +1 more
TCPOBOP appears to have diminished potency and intrinsic activity in the rat, but it does not antagonize the effect of phenobarbital (i.e., act as a partial agonist), and in four strains of mice and Syrian golden hamsters, TCPOBOP was a potent inducer of hepatic aminopyrine N -demethylase activity, but four strain of rats and guinea pigs failed to respond.
Journal of Molecular BiologyClustering of the DNA sequences complementary to repetitive nuclear RNA of HeLa cells
72 Citations1975Marialuisa Melli, Enrico Ginelli +2 more
The hybridization profile of heterogeneous nuclear RNA from HeLa cells across DNA density gradients is studied, and it is concluded that the repeated sequences in HnRNA are complementary to clusters of repeats in the DNA.
Proceedings of the National Academy of SciencesIsolation and characterization of a cloned DNA sequence associated with the murine <i>Ah</i> locus and a 3-methylcholanthrene-induced form of cytochrome <i>P</i> -450
72 Citations1981Masahiko Negishi, David C. Swan +2 more
Data demonstrate conclusively that pBR322 clone 46 DNA is associated with mRNA controlled by the murine Ah locus, presumably the structural gene encoding 3-methylcholanthrene-induced P(1)-450.