The Mad2 partial unfolding model: regulating mitosis through Mad2 conformational switching
The Journal of Cell BiologyPublished 24 November 2008Open access
John J. Skinner, Stacey Wood, James Shorter, S. Walter Englander, Ben E. Black
Citations56
SJR quartileQ1
SJR score3.36
SNIP1.52
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TL;DR
The metamorphic Mad2 protein acts as a molecular switch in the checkpoint mechanism that monitors proper chromosome attachment to spindle microtubules during cell division, and catalyzed conversion requires a major structural rearrangement that transits through a partially unfolded intermediate.
Abstract
The metamorphic Mad2 protein acts as a molecular switch in the checkpoint mechanism that monitors proper chromosome attachment to spindle microtubules during cell division. The remarkably slow spontaneous rate of Mad2 switching between its checkpoint inactive and active forms is catalyzed onto a physiologically relevant time scale by a self-self interaction between its two forms, culminating in a large pool of active Mad2. Recent structural, biochemical, and cell biological advances suggest that the catalyzed conversion of Mad2 requires a major structural rearrangement that transits through a partially unfolded intermediate.
Keywords
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