Amino acid sequence of a chicken heat shock protein derived from the complementary DNA nucleotide sequence
BiochemistryPublished 7 October 1986
Markku S. Kulomaa, Nancy L. Weigel, Don A. Kleinsek, Wanda G. Beattie, Orla M. Conneely, Carl J. March
Citations77
SJR quartileQ1
SJR score1.18
SNIP0.73
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
Abstract
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAmino acid sequence of a chicken heat shock protein derived from the complementary DNA nucleotide sequenceMarkku S. Kulomaa, Nancy L. Weigel, Don A. Kleinsek, Wanda G. Beattie, Orla M. Conneely, Carl March, Tanya Zarucki-Schulz, William T. Schrader, and Bert W. O'MalleyCite this: Biochemistry 1986, 25, 20, 6244–6251Publication Date (Print):October 7, 1986Publication History Published online1 May 2002Published inissue 7 October 1986https://pubs.acs.org/doi/10.1021/bi00368a061https://doi.org/10.1021/bi00368a061research-articleACS PublicationsRequest reuse permissionsArticle Views56Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
Proceedings of the National Academy of SciencesDNA sequencing with chain-terminating inhibitors
69,306 Citations1977Frederick Sanger, S. Nicklen +1 more
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal of Molecular BiologyDetection of specific sequences among DNA fragments separated by gel electrophoresis
32,820 Citations1975E.M. Southern
This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Nucleic Acids ResearchA rapid alkaline extraction procedure for screening recombinant plasmid DNA
14,877 Citations1979H.C. Birnboim, Janine Doly
The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Proceedings of the National Academy of SciencesA new method for sequencing DNA.
7,969 Citations1977Allan M. Maxam, Wendy V. Gilbert
Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Proceedings of the National Academy of SciencesPurification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
6,888 Citations1972Haim Aviv, Philip Leder
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.
GeneA simple and very efficient method for generating cDNA libraries
4,564 Citations1983Ueli Gubler, Beth J. Hoffman
Using the fully sequenced 1300 nucleotide-long bovine preproenkephalin mRNA, it is established by sequencing that the method yields faithful full-length transcripts.
BiochemistryRNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination
3,481 Citations1977Hans Lehrach, Don J. Diamond +2 more
RNA molecular weight measurements were carried out by gel electrophoresis under four different denaturing conditions including 99% formamide, 10 mM methyl mercury, 2.2 M formaldehyde, and 6 M urea at pH 3.8 to demonstrate reliable molecular weight determinations of denatured RNAs, especially those obtained by extrapolation.
CellSizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
3,010 Citations1977Arnold Berk, Phillip A. Sharp
A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
Proceedings of the National Academy of SciencesEfficient isolation of genes by using antibody probes.
2,058 Citations1983R A Young, Ryan Davis
A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.
CellThe isolation of structural genes from libraries of eucaryotic DNA
1,856 Citations1978Tom Maniatis, Ross C. Hardison +6 more
A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.
ScienceRat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
1,254 Citations1977Axel Ullrich, John Shine +5 more
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA that contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin II prepeptide, and the untranslated 3' terminal region of the mRNA.
ScienceYeast RNA Polymerase II Genes: Isolation with Antibody Probes
1,078 Citations1983Richard A. Young, Ronald W. Davis
The identity of genes that specify the largest RNA polymerase II subunits, the 220-000- and 150,000-dalton polypeptides, was confirmed by competitive radioimmune assay.
Nucleic Acids ResearchThe ovalbumin gene - sequence of putative control regions
999 Citations1980Christophe Benoıst, K. O'Hare +2 more
The sequence of regions of the chicken ovalbumin gene believed to be important in the control of initiation of transcription, splicing, and transcription termination or polyadenylation are presented.
CellAnalysis of transcriptional regulatory signals of the HSV thymidine kinase gene: Identification of an upstream control region
646 Citations1981Steven L. McKnight, Elizabeth R. Gavis +2 more
It appears that the sequences between 16 and 32 nucleotides upstream from the 5' terminus of the tk gene are required to specify the exact start site of transcription, however, control of both the efficiency of transcription and rough specification of the position of initiation depends on sequences 40--100 nucleotide upstream fromthe tk structural gene.
Proceedings of the National Academy of SciencesIsolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
569 Citations1980David A. Goldberg
The alcohol dehydrogenase (ADH) gene (Adh) of Drosophila melanogaster was isolated by utilizing a mutant strain in which the Adh locus is deleted, and two intervening sequences were identified within Adh by S1 nuclease mapping experiments.
Journal of BacteriologyStimulation by Cyclic Adenosine Monophosphate of Plasmid Deoxyribonucleic Acid Replication and Catabolite Repression of the Plasmid Deoxyribonucleic Acid-Protein Relaxation Complex
505 Citations1973Leonard Katz, David T. Kingsbury +1 more
BiochemistryPreparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct
222 Citations1975Jeffrey M. Rosen, Savio L.C. Woo +3 more
The size of the ovalbumin mRNA is considerably greater than that required to code for a protein of 387 amino acids, which should now permit a more thorough analysis of its physical and chemical properties.
CellStudies of cloned sequences from four drosophila heat shock loci
199 Citations1979Robert A. Holmgren, Kenneth J. Livak +3 more
DNA cloned from the D. melanogaster heat shock loci at 63BC and 95D codes for the 83,000 and the 68,000 dalton heat shock proteins, respectively, and considerable homology is found between these sequences and a site in 87D.
Journal of Molecular BiologyNucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B
197 Citations1983Richard Southgate, Agnes Ayme +1 more
Investigation of the hydrophilic/hydrophobic characteristics of the four polypeptides of Drosophila melanogaster revealed the presence of an alpha-helical region of very prominent local hydrophilicity, which probably represents a surface structural domain common to each protein.
Journal of Biological ChemistryComplete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae.
187 Citations1984Frances W. Farrelly, David Finkelstein
Although the sequences surrounding the coding region of the HSP90 gene are quite similar to those surrounding other sequenced yeast genes, the authors find a limited homology between sequences located 5' to this gene and the putative heat shock-regulatory sequences located5' to the heatshock-inducible genes of Drosophila.
Methods in enzymology on CD-ROM/Methods in enzymology[59] Determination of RNA sequences by primer directed synthesis and sequencing of their cDNA transcripts
137 Citations1980P K Ghosh, V.B. Reddy +3 more
This chapter discusses the determination of RNA Sequences by primer directed synthesis and sequencing of their cDNA transcripts by the method described in the chapter.
European Journal of BiochemistryThe N-Terminal Amino-Acid Sequences of DNA Polymerase I from Escherichia coli and of the Large and the Small Fragments Obtained by a Limited Proteolysis
121 Citations1974Henning Jacobsen, Hans Klenow +1 more
The two fragments obtained by proteolytic cleavage of DNA polymerase I from Escherichia coli have been isolated by chromatography on hydroxyapatite and shows unambiguously that the small fragment is placed in the N-terminal end of the native enzyme.
Journal of Biological ChemistryInteraction of bacteriophage T4 RNA and DNA ligases in joining of duplex DNA at base-paired ends.
117 Citations1977Akio Sugino, H. M. Goodman +4 more
The joining of duplex DNA at base-paired ends by bacteriophage T4DNA ligase was confirmed using either a synthetic duplex decamer or restriction endonuclease fragments of ColE1 DNA as substrates and RNA ligase only moderately affected the joining of cohesive ends by T4 DNA ligase or E. coli DNAligase.
Biochemical and Biophysical Research CommunicationsPhosphorylation of hen progesterone receptor by cAMP dependent protein kinase
111 Citations1981Nancy L. Weigel, Joseph S. Tash +3 more
Progesterone receptor A and B subunits from laying hen oviducts were highly purified and their phosphorylation by cAMP-dependent protein kinase from bovine heart was studied, indicating that the receptors are good substrates.
Proceedings of the National Academy of SciencesRapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.
78 Citations1979Minou Bina-Stein, Marilyn M. Thorén +2 more
Using specific DNA restriction fragments as primers in combination with this technique, the sequence of the spliced junction between the body and the leader sequence ofThe 16S late mRNA of simian virus 40 is determined.
Journal of Molecular BiologySteroid and high-temperature induction of the small heat-shock protein genes in Drosophila
68 Citations1984Michael P. Vitek, Edward M. Berger
One intriguing finding is that hsp message stability is temperature-dependent; hsp transcripts are two to three times more stable at 35 degrees C than at 25 degrees C; the possible mechanism and significance of this phenomenon are discussed.
Nucleic Acids ResearchCloning of the chick hsp 90 cDNA in expression vector
56 Citations1985M.G. Catelli, Nadine Binart +2 more
Southern blot analysis of chicken genomic DNA indicated that the cDNA hybridizes to a single copy sequence, and Sequence data show that the p9.11 cDNA displays a high degree of homology with the 5' portion of yeast heat shock protein 90 cDNA.
Journal of Biological ChemistryThe putative molybdate-stabilized progesterone receptor subunit is not a steroid-binding protein.
39 Citations1984M Birnbaumer, Richard Bell +2 more
It is shown that the Mr = 88,000 +/- 2,000 protein is not a receptor but is apparently an artifact of the use of particular steroid affinity resins.
BiochemistryStructural analysis of chicken oviduct progesterone receptor using monoclonal antibodies to the subunit B protein
39 Citations1984Dean P. Edwards, Nancy L. Weigel +3 more
Two monoclonal antibodies against the B subunit (Mr 108 000) of chick oviduct progesterone receptor (PgR) were produced by immunizing rats and fusing spleen cells with NS-1 mouse myeloma cells, and antibody-receptor interactions were demonstrated under protein denaturing conditions.
Proceedings of the National Academy of SciencesMolecular cloning of a cDNA for the chicken progesterone receptor B antigen.
24 Citations1984Tanya Zarucki-Schulz, M.S. Kulomaa +7 more
Preliminary studies show that withdrawal of hormone from chickens treated chronically with estrogen leads to a dramatic decrease in the cellular RNA concentration of receptors B, indicating that target tissue levels of receptor B RNA are under hormonal control.
Journal of Biological ChemistryImmunologic detection of a protein homologous to chicken progesterone receptor B subunit.
17 Citations1985Sara Peleg, William T. Schrader +3 more
It is found that the antibody does not recognize the form of receptor B able to bind [3H]progesterone in vitro, and reacts exclusively with a homologous protein of the same molecular weight, termed B antigen, which is present in both immature estrogen-treated chicks and in egg-laying hens.