The Single-Stranded DNA Phages
CRC Critical Reviews in MicrobiologyPublished 1 January 1975
David T. Denhardt, Peter Model
Citations296
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Abstract
(1975). The Single-Stranded DNA Phages. CRC Critical Reviews in Microbiology: Vol. 4, No. 2, pp. 161-223.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
Journal of Biological ChemistryThe deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.
324 Citations1975J. J. M. Weiner, LL Bertsch +1 more
The DNA unwinding protein of Escherichia coli has been purified to homogeneity by a simple procedure which utilizes its stability to heating and depends on its essential role in the conversion of phage G4 single-stranded DNA to the replicative form.
Proceedings of the National Academy of SciencesRNA Synthesis Initiates <i>In Vitro</i> Conversion of M13 DNA to Its Replicative Form
301 Citations1972William Wickner, Douglas L. Brutlag +2 more
Rifampicin does not block synthesis of the replicative forms of varphiX174 either in vivo or in soluble extracts, and DNA synthesis with deoxyribonucleoside triphosphates and a fresh addition of enzymes is not required.
Journal of Molecular BiologyIsolation and characterization of gene 5 protein of filamentous bacterial viruses
292 Citations1972Bruce Alberts, Linda Frey +1 more
Journal of Biological ChemistrydnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.
233 Citations1975Jean‐Pierre Bouché, Kasper Zechel +1 more
The protein responsible for the initiation of conversion of single-stranded phage G4 DNA to the duplex replicative form has been purified approximately 3000-fold and identified with Escherichia coli dnaG gene product, a rifampicin-resistant RNA polymerase of approximately 64,000 daltons.
ScienceMultienzyme Systems of DNA Replication
220 Citations1974Randy Schekman, Alan L. Weiner +1 more
Understanding of the regulation of replication events in the cell, their localization at membrane surfaces and integration with cell division, and their coordination with phage DNA maturation and particle assembly will all be advanced by knowledge of the components of the replicative machinery.
Proceedings of the National Academy of SciencesNucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.
211 Citations1975Heinz Schaller, Christopher Gray +1 more
The primary structure of a strong RNA polymerase binding site in the replicative form DNA of phage fd has been determined by direct DNA sequencing and it is: the molecule contains regions with 2-fold symmetry and sequence homologies to promoter regions from other DNAs.
Journal of VirologySpecific Fragments of φX174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from <i>Haemophilus aegyptius</i> , Endonuclease Z
206 Citations1972June H. Middleton, Marshall H. Edgell +1 more
A restriction-like enzyme has been purified from Haemophilus aegyptius that produces a rapid decrease in the viscosity of native calf thymus and H. influenzae deoxyribonucleic acids, but does not degrade homologous DNA.
Journal of Molecular BiologyThe rep mutation
200 Citations1975H. E. D. Lane, David T. Denhardt
It is concluded that in rep mutants the speed of replication fork movement is only 50 to 60% of that of wild-type cells.
Journal of Biological ChemistryConversion of the M13 Viral Single Strand to the Double-stranded Replicative Forms by Purified Proteins
199 Citations1974Klaus Geider, Arthur Kornberg
It is shown that the conversion of viral M13 DNA to the covalently closed duplex form requires the action of six purified proteins: RNA polymerase, DNA unwinding protein of Escherichia coli, DNA polymerase III*, copolymerase III**, DNA Polymerase I, and DNA ligase.
Proceedings of the National Academy of SciencesInitiation of DNA Synthesis: Synthesis of ϕX174 Replicative Form Requires RNA Synthesis Resistant to Rifampicin
191 Citations1972Randy Schekman, William Wickner +5 more
It is presumed that, as in the case of M13, synthesis of a short RNA chain primes the synthesis of an replicative form by DNA polymerase, and initiation of DNA synthesis by RNA priming is a mechanism of wide significance.
Journal of Molecular BiologyDNA synthesis in nucleotide-permeable Escherichia coli cells
186 Citations1971Hans-Peter Vosberg, Hartmut Hoffmann‐Berling
DNA synthesis was studied in endonuclease I (endI)-deficient mutant cells, made permeable to nucleotides by a short treatment with ether to indicate that ether-treated cells contain unfragmented DNA under normal assay conditions.
Proceedings of the National Academy of SciencesConversion of ϕX174 Viral DNA to Double-Stranded Form by Purified <i>Escherichia coli</i> Proteins
174 Citations1974Sue Wickner, Jerard Hurwitz
The E. coli proteins that catalyze the conversion of varphiX174 single-stranded DNA to duplex DNA have now been purified extensively and DNA synthesis by these proteins requires varphi X174 viral DNA, dNTPs, Mg(+2), and ATP.
Journal of Molecular BiologyProperties of the isolated gene 5 protein of bacteriophage fd
165 Citations1972Jan L. Oey, Rolf Knippers
Gene 5 protein of bacteriophage fd was purified from phage infected bacteria and increases the template activity of a viral replicative form DNA for polymerase II at low concentrations.
Journal of Molecular BiologyIn vitro synthesis of bacteriophage f1 proteins
150 Citations1974Peter Model, Norton D. Zinder
Covalently closed, circular, double-stranded DNA isolated from cells infected with bacteriophage f1 has been used as a template for coupled transcription and translation in vitro and it has been possible to identify six gene-specific polypeptides.
Journal of Molecular BiologyGenetic control of bacteriophage M13 DNA synthesis
147 Citations1968David Pratt, William S. Erdahl
The experiments show that no viral protein synthesis is needed to permit conversion of the infecting DNA strand to an RF molecule, but that the product of M13 gene 2 is required for the build-up of the RF pool and theProduct of gene 5 for progeny single-strand production.
Journal of Molecular BiologyRole of coliphage M13 gene 5 in single-stranded DNA production
147 Citations1971John S. Salstrom, David Pratt
The phage M13 gene 5 protein is shown to play a negative role in the production of viral single-stranded DNA.
Journal of Biological ChemistryDeoxyribonucleic Acid Synthesis in Cell-free Extracts
146 Citations1972Thomas B. Kornberg, Malcolm L. Gefter
DNA polymerase III, an enzyme essential for DNA replication, has been purified more than 10,000-fold from cell-free extracts of Escherichia coli and is present in amounts sufficient to account for the in vivo rate of replication.
VirologyCharacterization of REP− mutants and their interaction with P2 phage
145 Citations1970Richard Calendar, Björn Lindqvist +2 more
Using REP− strains derived by transduction, it is obtained no evidence that three rep mutations confer recombination deficiency, although they do do appear to confer a slight increase in UV sensitivity.
CRC Critical Reviews in MicrobiologyThe Properties of Sex pili, The Viral Nature of “Conjugal” Genetic Transfer Systems, and Some Possible Approaches to the Control of Bacterial Drug Resistance
141 Citations1971Charles C. Brinton, Louis S. Baron
Journal of Biological ChemistryTen proteins required for conversion of phiX174 single-stranded DNA to duplex form in vitro. Resolution and reconstitution.
140 Citations1975Randy Schekman, Joël H. Weiner +2 more
Protein requirements for conversion of phiX174 single-Stranded DNA to a double-stranded replicative form with a small gap (RF II) have been determined by resolution and reconstitution of the multienzyme system from extracts of gently lysed Escherichia coli.
VirologyConditional lethal mutants of the small filamentous coliphage M13
139 Citations1969David Pratt, Helen Tzagoloff +1 more
These results, together with results published elsewhere, show that gene 3 codes for the phage attachment protein, probably present as a single subunit at one end of the filamentous virion, while gene 8 code for the major coat protein subunit.
Journal of Molecular BiologyFilamentous bacterial viruses
133 Citations1974D.A. Marvin, R.L. Wiseman +1 more
X-ray diffraction patterns of the fd, If1 and IKe strains of filamentous bacterial viruses (class I) indicate that the arrangement of capsid proteins in the virion approximates a left-handed helix of 15 A pitch with 4.5 units per turn.
Journal of Molecular BiologyDirect determination of DNA nucleotide sequences: Structure of a fragment of bacteriophage φX174 DNA
132 Citations1974Francis Galibert, John W. Sedat +1 more
Methods for the direct determination of nucleotide sequences in DNA have been developed and used to determine the complete primary structure of a fragment of bacteriophage φX174 DNA which is 48 residues in length.
Proceedings of the National Academy of SciencesA New Form of DNA Polymerase III and a Copolymerase Replicate a Long, Single-Stranded Primer-Template
131 Citations1973William Wickner, Randy Schekman +2 more
A new form of DNA polymerase III, termed Pol III star (Pol III(*)), has been purified to homogeneity from Escherichia coli and is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III.
Journal of VirologyGenetic Map of Bacteriophage φX174
131 Citations1971Robert M. Benbow, Clyde A. Hutchison +2 more
Bacteriophage φX174 temperature-sensitive and nonsense mutations in eight cistrons were mapped by using two-, three-, and four-factor genetic crosses, consistent with a small closed circular deoxyribonucleic acid molecule as a genome.
Journal of Biological ChemistryPurification and Properties of the Escherichia coli Deoxyribonucleic Acid-unwinding Protein
123 Citations1974Ian J. Molineux, Susan Friedman +1 more
A possible physiological role for DNA polymerase II and the unwinding protein of E. coli is suggested to be essential for the synthesis of a DNA product that is equal in size to the template.
Journal of Molecular BiologyComplex of bacteriophage M13 single-stranded DNA and gene 5 protein
119 Citations1974David Pratt, Peter Laws +1 more
It is assumed that the complex found in lysates of infected cells previously did exist as such inside the cells, but it is unable to exclude that it formed during or after lysis, and the results of pulse-chase radioactive labeling experiments on infected cells can be interpreted as showing that with time the single-stranded DNA leaves complex, presumably to be matured into virions, while the gene 5 proteins are re-used to form more complex.
Journal of Biological ChemistryInteraction of deoxycholate and of detergents with the coat protein of bacteriophage f1
108 Citations1975S. Makino, John L. Woolford +2 more
The major coat protein of bacteriophage f1, which is localized in the host membrane during phage maturation, has a hydrophobic binding site capable of binding deoxycholate and a variety of detergents to form a soluble particle, and in that respect, resembles many membrane proteins.
Proceedings of the National Academy of SciencesTHE PROTEINS OF BACTERIOPHAGE M13
108 Citations1969Timothy J. Henry, David Pratt
Phage proteins are synthesized at nearly normal rates in cells in which replication of phage double-stranded DNA is blocked by gene 2 mutations, suggesting that the initial double- Stranded DNA molecule serves as the principal template, perhaps the only template, for phage messenger RNA synthesis.
Journal of VirologySpecific Endonuclease R Fragments of Bacteriophage φX174 Deoxyribonucleic Acid
102 Citations1972Marshall H. Edgell, Clyde A. Hutchison +1 more
Using the genetic assay for small fragments of phiX DNA, it is shown that it can salvage markers from the endonuclease R phiZ174 replicative-form deoxyribonucleic acid fragments.
Proceedings of the National Academy of SciencesAssociation of DNA-dependent and -independent Ribonucleoside Triphosphatase Activities with <i>dnaB</i> Gene Product of <i>Escherichia coli</i>
101 Citations1974Sue Wickner, Michel Wright +1 more
Acrylamide gel electrophoresis of the purified material shows a single band of protein coincident with eluted dnaB complementing and DNA-dependent and -independent nucleoside triphosphatase activities.
Proceedings of the National Academy of SciencesDNA Polymerase III Star Requires ATP to Start Synthesis on a Primed DNA
100 Citations1973William Wickner, Arthur Kornberg
ATP and copolymerase III star are required to initiate chain growth but not to sustain it, as ADP and inorganic phosphate are discharged and chain elongation is resistant to antibody toCopolymerases III star.
Journal of Molecular BiologyFormation of the parental replicative form DNA of bacteriophage φX174 and initial events in its replication
100 Citations1971Bertold Francke, Dan S. Ray
It is concluded that the virus-specified discontinuity in RFII is introduced after the first complementary strand is completed and closed and represents the result of the action of the gene A product on the DNA.
Journal of Molecular BiologyGenetic studies with heteroduplex DNA of bacteriophage f1. Asymmetric segregation, base correction and implications for the mechanism of genetic recombination
99 Citations1975Vincenzo Enea, Gerald P. Vovis +1 more
Heteroduplex DNA of bacteriophage f1 constructed in vitro was used to transfect Escherichia coli and shows that one strand—usually the minus strand—determines in large part the genotypes of progeny phage.
BiochemistryTemplate activities of the Φη-174 replicative allomorphic deoxyribonucleic acids
99 Citations1971Yukimasa Hayashi, Masaki Hayashi
Proceedings of the National Academy of SciencesIsolation and Function of the Gene <i>A</i> Initiator of Bacteriophage ϕX 174, A Highly Specific DNA Endonuclease
99 Citations1974Timothy J. Henry, Rolf Knippers
The gene A product of Escherichia coli bacteriophage varphiX 174, necessary for initiation ofvarphiX DNA synthesis, has been identified and purified to near homogeneity and is shown to be a highly specific DNA endonuclease which breaks one phosphodiester bond in the viral strand.
Proceedings of the National Academy of SciencesCleavage of Bacteriophage f1 DNA by the Restriction Enzyme of <i>Escherichia coli</i> B
96 Citations1972Kensuke Horiuchi, Norton D. Zinder
The results indicate that Circular replicative forms are broken once to yield full-length linear molecules (RF III), which are less susceptible than RF I to endonuclease R-B to B-restriction.
Journal of Biological ChemistryReplication of phage G4. A novel and simple system for the initiation of deoxyribonucleic acid synthesis.
95 Citations1975Kasper Zechel, Jean‐Pierre Bouché +1 more
Stimulation by ATP and GTP suggests an RNA synthetic step in the priming of DNA synthesis, which depends on a novel and relatively simple group of three proteins.
Journal of BacteriologyRetraction of F Pili
94 Citations1974Charles P. Novotny, Paula Fives‐Taylor
The disappearance of F pili on Escherichia coli cells in the presence of 10(-2) M NaCN was studied by electron microscopy and serum-blocking power and suggests that the tips of retracted pili are exposed.
VirologyStages in phage R17 infection
89 Citations1972P.M. Krahn, Richard J. O’Callaghan +1 more
Phage R17 was specifically labeled in the A protein with 3H-histidine, and in the RNA with 32P, and it was suggested that the penetration of phage RNA into the host cell may involve the injection of an A protein-RNA complex rather than free RNA.
Journal of Molecular BiologyRegulation of coliphage f1 single-stranded DNA synthesis by a DNA-binding protein
88 Citations1973Babbaka J. Mazur, Peter Model
It is proposed that this accumulated gene 5 protein, by binding to the limited number of replicating DNA molecules formed following the shift to the permissive temperature, acts to prevent the synthesis of double-stranded replicative form DNA, thus causing the predominant appearance of single strands.
Journal of Molecular BiologyReplication of bacteriophage M13
87 Citations1969Dan S. Ray
Sucrose-gradient sedimentation of DNA from cells pulse-labeled with tritiated thymidine at 130 minutes after M13 infection shows a flow of material from RF ‡ to progeny single strands, which is found in both viral and complementary strands of RF only after long-term labeling from the time of infection.
Journal of Molecular BiologyThe mechanism of replication of φX174 single-stranded DNA
87 Citations1971Randy Schekman, Makoto Iwaya +2 more
Observations suggest that RFII is first synthesized as a molecule with a gap in one strand bounded by a 5′-phosphate and a 3′-hydroxyl, which may be converted to RFI by the action first of E. coli DNA polymerase then of polynucleotide ligase.
Journal of Molecular BiologyConformations of single-stranded DNA and coat protein in fd bacteriophage as revealed by ultraviolet absorption spectroscopy
86 Citations1969Loren A. Day
According to criteria for distinguishing the effects of spectral shifts and changes in absorption intensity on difference spectra, the spectrum for intact phage shows significant hyperchromicity centered around 290 mμ relative to the spectra of separated DNA and protein.
Journal of BacteriologyRole of Pili in Bacterial Conjugation
86 Citations1970Jonathan T. Ou, Thomas F. Anderson
It is concluded that F pili play at least three roles in mating: (i) they initiate contacts between mating pairs; (ii) they facilitate the transfer of genetic material; and (iii) they draw mating cells into a close contact which increases the fertility of the union.
Journal of Molecular BiologyFilamentous bacterial viruses
82 Citations1974D.A. Marvin, W.J. Pigram +3 more
Journal of Molecular BiologyElectrophoretic characterization of φX174-specific proteins
82 Citations1969David H. Gelfand, Masaki Hayashi
By infecting u.v.-irradiated hosts with amber mutants in each of seven complementation groups, an acrylamide gel electrophoresis assay for φX174-specific proteins synthesized in vivo is described.
Journal of Molecular BiologyThe process of infection with bacteriophage φX174
82 Citations1970John E. Newbold, Robert L. Sinsheimer
Journal of VirologyBacteriophage f1 Infection: Fate of the Parental Major Coat Protein
81 Citations1974Henry M. Smilowitz
The major coat protein of infecting f1 phage is incorporated into the inner membrane of the host cell, even in the absence of phage f1DNA penetration and replication.
Journal of BacteriologyThe<i>rep</i>Mutation III. Altered Structure of the Replicating<i>Escherichia coli</i>Chromosome
81 Citations1974H. E. D. Lane, David T. Denhardt
The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA) and it is deduced that the rep function is required for the "normal" replication of the E. coli chromosome and that in its absence the E coli chromosome replicates in an altered manner, perhaps involving slower-moving replicating forks.
Journal of Biological ChemistryGene V protein of fd bacteriophage. Dimer formation and the role of tyrosyl groups in DNA binding.
81 Citations1975HT Pretorius, Michèle Klein +1 more
It is postulated that tyrosyl groups are not involved in the protein-protein interactions of the monomer-dimer equilibrium, that tyrosine groups stack with DNA bases in the complexes, and that each subunit of gene V protein in the intracellular complexes with fd-DNA is replaced by exactly two subunits of major coat protein during final assembly of the virus.
Journal of Molecular BiologyThe mechanism of replication of øX174 DNA
80 Citations1975Shlomo Eisenberg, Barbara Harbers +2 more
It is suggested that synthesis of all viral DNA during oX RF replication is initiated at non-randomly distributed sites on the oX genome.
Journal of Molecular BiologySequence and symmetry in ribosome binding sites of bacteriophage f1 RNA
79 Citations1974George Pieczenik, Peter Model +1 more
RNA synthesized in vitro from α-32P-labeled ribonucleoside triphosphates with Escherichia coli RNA polymerase from covalently closed, circular, double-stranded DNA isolated from cells infected with bacteriophage f1 is found to fall into three classes.
Journal of VirologyGenetic Assay for Small Fragments of Bacteriophage φX174 Deoxyribonucleic Acid
79 Citations1971Clyde A. Hutchison, Marshall H. Edgell
This method is potentially useful as a specific assay to aid in the purification of genetically defined DNA fragments and also as a mechanism for the incorporation of small chemically synthesized DNA sequences into viral genomes.
VirologyMini M13 bacteriophage: Circular fragments of M13 DNA are replicated and packaged during normal infections
78 Citations1974Jack D. Griffith, Arthur Kornberg
Analysis of mini phage DNA by heteroduplex electron microscopy showed them to be fragments of the M13 circle containing the unique site for a restriction endonuclease and extending for a variable distance in one direction from this site.
VirologyEvolution of φX174. Isolation of four new φX-like phages and comparison with φX174
77 Citations1974G. Nigel Godson
Four new single-stranded circular DNA-containing icosahedral phages have been isolated using a sucrose density gradient to fractionate the phages present in raw sewage into size classes, and every protein coded by the G phages has a recognizable φX174 counterpart of approximately the same molecular weight.
NaturePrimary structure and sidechain interactions of PFL filamentous bacterial virus coat protein
77 Citations1975Yoshiki Nakashima, R.L. Wiseman +2 more
The first system for which a molecular model of a structural protein has been described in sufficient detail to permit study of the bonding specificity between proteins is described, finding that α helices are arranged in the virion so that hydrophobic sidechains on each protein subunit can fit into the space between sidechain on neighbouring subunits.
Proceedings of the National Academy of Sciences<i>Cis</i> -Limited Action of the Gene-A Product of Bacteriophage ϕX174 and the Essential Bacterial Site
75 Citations1972Bertold Francke, Dan S. Ray
Double-infection experiments with wild-type phage and phage carrying an amber mutation in gene A show that the formation of gene A-specific RFII and RI is cis-limited to only the wild- type DNA, providing a basis at the DNA level for the known asymmetric complementation of genes A.
VirologyBinding, eclipse, and penetration of the filamentous bacteriophage M13 in intact and disrupted cells
74 Citations1974Roberto Di Marco, S. Michal Jazwinski +1 more
Current models for the key role of the F pilus (of F+ cells) in phage adsorption are discussed and a larger role for the phage advertisersorption protein is proposed.
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisReplication of bacteriophage M13
74 Citations1969Dan S. Ray, Randy Schekman
Velocity sedimentation at high ionic strength, of DNA released from M13-infected bacteria lysed under gentle conditions, provides a simple method for separating phage-specific DNA components from bacterial DNA.
Journal of VirologyIdentification and characterization of the in vitro synthesized gene products of bacteriophage M13
74 Citations1975R.N.H. Konings, Theo J.M. Hulsebos +1 more
Results suggest that the latter proteins arise in vivo by cleavage of precursor molecules more than twice as long as their native counterparts present within the virion.
Journal of Biological ChemistryInitiation of Deoxyribonucleic Acid Synthesis
73 Citations1973Ole Westergaard, Douglas L. Brutlag +1 more
The product of in vitro replication of the ΦX174 or M13 viral strand is known to be a circular duplex with a discontinuity in the synthetic strand (RF II), which has been converted to an alkali-stable covalent duplex (RF I) through the joint action of Escherichia coli DNA polymerase I and E. coli ligase.
Journal of Molecular BiologyReplication of bacteriophage M13
70 Citations1972Hovsép Mgedich Fidanián, Dan S. Ray
The formation of a viral-strand specific discontinuity in M13 replicative form DNA was observed in Escherichia coli C-1704 rep − 3, a bacterial mutant that allows replicativeform DNA formation but not its replication.
Nature New BiologySingle RNA Polymerase Binding Site Isolated
69 Citations1972Bertold Heyden, Christiane Nüsslein +1 more
The isolation of a single RNA polymerase binding site from the replicative form (RF) DNA of bacteriophage fd is reported, which is a short double-stranded DNA with a unique nucleotide sequence.
Journal of Molecular BiologyStructure of the DNA of bacteriophage fd
68 Citations1969Heinz Schaller, Hartmut Voss +1 more
A double strand-like DNA core has been detected in the single-stranded DNA of bacteriophage fd, which has resistance to degradation by single strand-specific nucleases, and is characterized by a high G + C-content, a simple pyrimidine distribution, and double strand -like spectral properties.
Journal of Molecular BiologyIntracellular location and number of replicating parental DNA molecules of bacteriophages lambda and φX174
68 Citations1969William O. Salivar, Robert L. Sinsheimerb
The parental DNA of bacteriophages λ and φX174 becomes associated with a host cell component after infection that has the properties of a lipoprotein and is probably the cell membrane.
Journal of Molecular BiologyRole of the gene B product in bacteriophage φX174 development
66 Citations1974Edward J. Siden, Masaki Hayashi
Proceedings of the National Academy of SciencesInvolvement of Two Protein Factors and ATP in <i>In Vitro</i> DNA Synthesis Catalyzed by DNA Polymerase III of <i>Escherichia coli</i>
65 Citations1974Jerard Hurwitz, Sue Wickner
These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA).oligo(dT) primer-template in the latter reaction, there is an absolute dependency on ATP or dATP.
Proceedings of the National Academy of SciencesKinetics of Methylation of DNA by a Restriction Endonuclease from <i>Escherichia coli</i> B
64 Citations1974Gerald F. Vovis, Kensuke Horiuchi +1 more
The restriction endonuclease from E. coli B is both an end onuclease and a DNA methylase, both of which require or are stimulated by Mg(+2), adenosine triphosphate, and S-adenosyl-L-methionine.
Journal of Molecular BiologyThe process of infection with bacteriophage φX174
63 Citations1968Arnold J. Levine, Robert L. Sinsheimer
A chloramphenicol-resistant, viral-directed protein, associated with the synthesis of progeny RF‡ in cells infected with bacteriophage φX174 has been isolated and a radioactive component preferentially present in the infected cell extracts was found.
Biochemical and Biophysical Research CommunicationsThe amino acid sequence of gene 5 protein of bacteriophage M 13
63 Citations1974Theo Cuypers, Frans J. van der Ouderaa +1 more
The amino acid sequence of gene 5 protein of bacteriophage M 13 was deduced by analysis of peptides derived from the protein by cleavage with cyanogen bromide and by digestion with trypsin and chymotrypsin.
BiochemistryMolecular weight of single-stranded fd bacteriophage DNA. High speed equilibrium sedimentation and light scattering measurements
63 Citations1974Steven A. Berkowitz, Loren A. Day
Journal of Molecular BiologyStarting nucleotide sequences of RNA synthesized on the replicative form DNA of coliphage fd
62 Citations1969Masahiro Sugiura, Toshio Okamoto +1 more
It was found that RNA chains synthesized on RF-I DNA were initiated predominantly with ATP- and GTP-ends, and it was concluded that at least three species of RNA starting with the following sequences were synthesized under the direction of RF- I DNA of phage fd: pppApUpGp-----, pppGpUpAp----- ppp GpUpUp-----.
Journal of Molecular BiologyAsymmetric information transfer during φX174 DNA replication
62 Citations1972Pieter Baas, H.S. Jansz
VirologyThe gene H spike protein of bacteriophages ∅X174 and S13
62 Citations1975S.Michael Jazwinski, Alf A. Lindberg +1 more
The actions in phage-receptor recognition and in transfection suggest that the gene H protein has multiple functions and behaves as a “pilot protein” in guiding the phage and its DNA through several stages of the infection cycle.
Journal of Molecular BiologyGenotypes produced by individual recombination events involving bacteriophage f1
62 Citations1971Thierry Boon, Norton D. Zinder
A breakage-copying model is proposed that accounts for the results and could also account for the general occurrence of both reciprocal and non-reciprocal recombination.
Journal of Molecular BiologyThe process of infection with bacteriophage φX174
61 Citations1969Marshall H. Edgell, Clyde A. Hutchison +1 more
Journal of Molecular BiologyStudies on the bacteriophage f1
60 Citations1968Edward F. Rossomando, Norton D. Zinder
Studies with the amber mutant R4 have shown that alterations in the gene-3 product decrease the stability of the phage and that therefore this cistron codes for the A protein, and the data suggest that the disassembly is an all-or-none phenomenon initiated by an ionization of a specific capsid protein.
Journal of Molecular BiologyRole of bacteriophage M13 gene 2 in viral DNA replication
60 Citations1972Norm S.-C. Lin, David Pratt
It is concluded that the gene 2 protein is involved in introducing a single-strand break into the covalently closed circular RFI† molecules, thus allowing them to replicate.
VirologyThe lipopolysaccharide receptor for bacteriophages ∅X174 and S13
58 Citations1975S. Michal Jazwinski, Alf A. Lindberg +1 more
Physical features of LPS imposed by its lipid components appear to be necessary for the succession of events that lead to eclipse, which may be the locus of the phage-binding site.
Journal of Molecular BiologyComparison of the effect of ultraviolet radiation and ethidium bromide intercalation on the conformation of superhelical φX174 replicative form DNA
58 Citations1973David T. Denhardt, Ann C. Kato
These experiments suggest that dimer formation and intercalation have the same effect on the structure of the DNA double helix, most probably that of partially unwinding the duplex.
BiochemistryHydrodynamic properties and molecular weight of fd bacteriophage DNA
57 Citations1974Jay Newman, Harry L. Swinney +2 more
VirologyThe adsorption of bacteriophage ΦX174 and its interaction with Escherichia coli; a kinetic and morphological study
57 Citations1972M. E. Bayer, Thomas W. Starkey
NatureThe nucleotide sequence of an RNA polymerase binding site on bacteriophage fd DNA
57 Citations1975Keiji Sugimoto, Toshio Okamoto +2 more
The nucleotide sequence of the RNA polymerase binding site at a promoter on fd RF DNA has been determined in comparison with the starting sequence of RNA initiated at this promoter.
Journal of VirologyDirection of Translation and Size of Bacteriophage φX174 Cistrons
57 Citations1972Robert M. Benbow, Robert F. Mayol +2 more
Translation of the bacteriophage phiX174 genome follows cistron order D-E-F-G-H-A-B-C, and distances on a physical map constructed from the molecular weights of phi X174 proteins and protein fragments are proportional over most of the genome.
NatureMechanism of Replication of φX-174 Single Stranded DNA
55 Citations1968David Dressler, David T. Denhardt
Progeny single stranded DNA seems to arise by displacement of plus strand material from precursor RF-type molecules during the asymmetric synthesis of newPlus strand material.
Journal of Molecular BiologyφX 174 replicative form DNA replication, origin and direction
54 Citations1972Pieter Baas, H.S. Jansz
Heteroduplex replicative form of bacteriophage φX174 was prepared by annealing minus strand isolated from wild-type φx RF with plus strand DNA, obtained from temperature-sensitive and amber mutants of φ X in different cistrons.
VirologyGene a of ΦX174
54 Citations1972Elwood Linney, Marie N. Hayashi +1 more
It is suggested that A′ protein is a product of the whole gene A while A protein is synthesized because of a natural internal initiator within the gene.
VirologySusceptibility of the ∅X-like phages G4 and G14 to R · EcoRI endonuclease
54 Citations1974G. Nigel Godson, Herbert W. Boyer
The cut by the R1 R-factor restriction enzyme (Eco RI) into unit length RF III linear DNA molecules of the new ΦX-like phages G4 and G14 is specific and located in the Haemophilus influenzae restriction enzyme fragments.
Journal of Molecular BiologyThe process of infection with bacteriophage φX174
52 Citations1969Rolf Knippers, William O. Salivar +2 more
Proceedings of the National Academy of SciencesA Cleavage Map of Bacteriophage ϕX174 Genome
52 Citations1974Amy S. Lee, Robert L. Sinsheimer
A detailed physical map of the ϕX174 genome has been constructed with respect to the cleavage sites of all three enzymes used to cleaveπX174 replicative form DNA into three sets of specific DNA fragments.
Journal of Molecular BiologyThe process of infection with bacteriophage φX174
51 Citations1969Arnold J. Levine, Robert L. Sinsheimer
Journal of Molecular BiologyEffect of cyanide and carbon monoxide on the replication of bacterial DNA in vivo
51 Citations1968John Cairns, David T. Denhardt
It is concluded that replication of the bacterial chromosome depends, at all times, upon some CO- and KCN-sensitive reaction which is not required, even in vivo, for the first steps in the synthesis of φX DNA.
Journal of VirologyEndonuclease R-EcoRII restriction of bacteriophage f1 DNA in vitro: ordering of genes V and VII, location of an RNA promotor for gene VIII
51 Citations1975Gerald F. Vovis, K Horiuchi +1 more
Replicative form DNA of bacteriophage f1 was found to be sensitive in vitro to restriction by endonuclease R-EcoRII if the DNA was isolated from an Escherichia coli strain deficient in cytosine methylase activity.
Journal of VirologyGenetic Recombination in Bacteriophage φX174
49 Citations1974Robert M. Benbow, Anthony J. Zuccarelli +2 more
Genetic recombination in bacteriophage phiX174 usually takes place early in the infection process and involves two parental replicative form (double-stranded) DNA molecules and an alternative, less efficient recombination mechanism is observed in the absence of the host recA protein.
Proceedings of the National Academy of SciencesA Coat Protein of the Bacteriophage M13 Virion Participates in Membrane-Oriented Synthesis of DNA
48 Citations1973S. Michal Jazwinski, Roberto Di Marco +1 more
It is suggested that the gene-3 protein may perform an essential function in the synthesis of replicative form by linking the phage DNA to a cellular replicative system in or on the inner cell membrane.
Journal of Molecular BiologyFilamentous bacterial viruses
48 Citations1971Barbara Höhn, Helga Lechner +1 more
The results are discussed in terms of an inhibitor model for the switch from double-strand replication to single-Strand synthesis and the abrupt decrease in acceleration of net DNA synthesis corresponds in time to the onset of synthesis of single-stranded viral DNA.
Nature New BiologyMultiple Length DNA Molecules of Bacteriophage φX174
46 Citations1972Robert M. Benbow, M Eisenberg +1 more
Molecular and General Genetics MGGTwo major groups of colicin factors: Their evolutionary significance
46 Citations1973K. G. Hardy, G. G. Meynell +2 more
Eleven colicin factors have been placed in two groups defined by a number of physiological criteria such as the effect of the host recA (recombination-proficiency) allele on colicIn titres and the maximum number of copies of the Colicin factor per chromosome.
Journal of VirologyMechanism of Adsorption and Eclipse of Bacteriophage φX174 II. Attachment and Eclipse with Isolated <i>Escherichia coli</i> Cell Wall Lipopolysaccharide
45 Citations1973Nino L. Incardona, LeRoy Selvidge
If the inactivation at 37 C is carried out on phiX-LPS complexes first formed at 15 C, the first-order kinetics are biphasic and nearly identical to that observed for the eclipse kinetics of phiZ attached to intact cells.
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