Identification of Ets- and Notch-Related Subunits in GA Binding Protein
SciencePublished 16 August 1991
Kelly LaMarco, Catherine C. Thompson, Brien P. Byers, Eve M. Walton, Steven L. McKnight
Citations350
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
Recombinant cDNA clones that encode two distinct subunits of the transcription factor GA binding protein have been isolated, revealing a conceptual convergence of nuclear transforming proteins and membrane-anchored proteins implicated in developmentally regulated signal transduction processes.
Abstract
Recombinant cDNA clones that encode two distinct subunits of the transcription factor GA binding protein (GABP) have been isolated. The predicted amino acid sequence of one subunit, GABP alpha, exhibits similarity to the sequence of the product of the ets-1 protooncogene in a region known to encompass the Ets DNA binding domain. The sequence of the second subunit, GABP beta, contains four 33-amino acid repeats located close to the NH2-terminus of the subunit. The sequences of these repeats are similar to repeats in several transmembrane proteins, including Notch from Drosophila melanogaster and Glp-1 and Lin-12 from Caenorhabditis elegans. Avid, sequence-specific binding to DNA required the presence of both polypeptides, revealing a conceptual convergence of nuclear transforming proteins and membrane-anchored proteins implicated in developmentally regulated signal transduction processes.
Keywords
Biochemistry, Genetics and Molecular Biology
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