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Con <sup>−</sup> Mutants: Class of Mutants in <i>Escherichia coli</i> K-12 Lacking a Major Cell Wall Protein and Defective in Conjugation and Adsorption of a Bacteriophage

Journal of BacteriologyPublished 1 September 1974Open access
Ronald A. Skurray, Robert E. W. Hancock, Peter R. Reeves
Citations193
SJR quartileQ2
SJR score1.09
SNIP0.85
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TL;DR

A new class of mutants of Escherichia coli K-12 defective in conjugation (Con(-) mutants), which lack a major protein of the outer membrane and are defective as recipients with Hfr and F' donors and as recipients for several F-like R factors and Col factors are described.

Abstract

We describe a new class of mutants of Escherichia coli K-12 defective in conjugation (Con(-) mutants). They lack a major protein of the outer membrane and are defective as recipients with Hfr and F' donors and as recipients for several F-like R factors and Col factors. In the case of crosses with an Hfr donor, we have shown that the Con(-) recipient is defective in pair formation. The mutants are resistant to certain phages due to loss of receptor activity.

Keywords

Biochemistry, Genetics and Molecular BiologyEnvironmental Science