The reaction site of a non‐competitive antagonist in the delta‐subunit of the nicotinic acetylcholine receptor.
The EMBO JournalPublished 1 August 1986Open access
Walter OBERTHÜR, Peter Muhn, Hugo Baumann, F. Lottspeich, B. Wittmann-Liebold, Ferdinand Hucho
Citations103
SJR quartileQ1
SJR score4.82
SNIP1.93
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TL;DR
A site in the primary structure of the nicotinic acetylcholine receptor from Torpedo marmorata covalently labeled with the non‐competitive antagonist [3H]triphenylmethylphosphonium (TPMP+) was localized and was found in position 262 of the delta‐polypeptide chain.
Abstract
A site in the primary structure of the nicotinic acetylcholine receptor from Torpedo marmorata covalently labeled with the non-competitive antagonist [3H]triphenylmethylphosphonium (TPMP+) was localized. The label was found in position 262 of the delta-polypeptide chain. This site is specifically labeled in the presence of the agonist carbamoylcholine. Labeling is prevented by the non-competitive antagonist histrionicotoxin. Position 262, probably a serine, is located in the highly conserved membrane-spanning helix M2 (according to the predicted folding scheme of Finer-Moore and Stroud (1984). The relationship of this site to the receptor's ion channel and its regulation is discussed.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
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