[12] DNA arrays for analysis of gene expression
Methods in enzymology on CD-ROM/Methods in enzymologyPublished 1 January 1999
Michael B. Eisen, Patrick O. Brown
Citations1,036
SJR quartileQ4
SJR score0.13
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TL;DR
The chapter focuses on the application of DNA microarrays to gene expression studies and discusses general principles of whole genome expression monitoring as well as detailing the specific process of making and using spotted DNAmicroarrays.
Abstract
This chapter describes one of the currently used microarray technologies commonly called “spotting” or “printing” because DNAs are physically spotted on a solid substrate in which short oligonucleotides is synthesized directly on a solid support. In standard spotting applications, large collections of DNA samples are assembled in 96- or 384-well plates. DNA microarrays are used for a variety of purposes; essentially any property of a DNA sequence that can be made experimentally to result in differential recovery of that sequence can be assayed for thousands of sequences at once by DNA microarray hybridization. The chapter focuses on the application of DNA microarrays to gene expression studies and discusses general principles of whole genome expression monitoring as well as detailing the specific process of making and using spotted DNA microarrays.
Keywords
Biochemistry, Genetics and Molecular Biology
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