Low-molecular-mass pI markers for isoelectric focusing
Journal of Chromatography APublished 1 February 1994
K. Sľais, Z. Friedl
Citations106
SJR quartileQ1
SJR score0.73
SNIP1.02
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Abstract
Substituted aminomethylphenols are proposed as low-molecular-mass pI markers for the electrophoretic and chromatographic focusing of ampholytes. The important acid-base, spectroscopic and hydrophobic characteristics are presented for nineteen synthesized compounds. The low interaction between the suggested pI markers and proteins was verified by gradient ion-exchange chromatography of a mixture of some markers and alcohol dehydrogenase.
Keywords
ChemistryEngineering
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