Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells.
Proceedings of the National Academy of SciencesPublished 1 October 1979Open access
Mark Achtman, Paul A. Manning, C. Edelbluth, Peter Herrlich
Citations166
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The proteins exported to the inner or outer membranes did not undergo proteolytic cleavage (processing) whereas beta-lactamase was processed normally.
Abstract
Most tra proteins encoded by the Escherichia coli F sex factor are incorporated into the minicell envelope. We have now assigned the tra proteins to cytoplasm (TraIp and 2b), inner membrane (TraEp, TraMp, and TraSp), and outer membrane (6e, TraAp, TraBp, TraJp, TraKp, TraLp, and TraTp). two proteins, TraDp and 6d, were associated with both inner and outer membranes. The proteins exported to the inner or outer membranes did not undergo proteolytic cleavage (processing) whereas beta-lactamase was processed normally.
Keywords
Biochemistry, Genetics and Molecular Biology
Journal of Biological ChemistryMechanism of Assembly of the Outer Membrane of Salmonella typhimurium
1,572 Citations1972Mary Osborn, J.E. Gander +2 more
FEBS LettersElectrophoretic resolution of the ‘major outer membrane protein’ of <i>Escherichia coli</i> K12 into four bands
1,419 Citations1975Ben Lugtenberg, Jos Meijers +3 more
Proceedings of the National Academy of SciencesNucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
783 Citations1978J G Sutcliffe
The nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase, is determined and the first 23 amino acids presumably form a signal for secretion.
Analytical BiochemistryAn efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli
392 Citations1976Bernard Witholt, Mieke Boekhout +4 more
This procedure has been tested on various strains of Escherichia coli at all stages of growth in minimal medium, from the early exponential to the late stationary phase, and very efficient conversion of cells to spheroplasts was observed in all cases.
Molecular and General Genetics MGGThe transposon Tn1 as a probe for studying ColE1 structure and function
298 Citations1977Gordon Dougan, David J. Sherratt
Insertion of the transposable genetic element Tn1 into different sites of plasmid ColE1 results in a number of mutant phenotypes, and non-transmissibility is correlated with reduced relaxation complex.
Proceedings of the National Academy of SciencesAmino acid sequence for the peptide extension on the prolipoprotein of the Escherichia coli outer membrane.
290 Citations1977S Inouye, Sen Wang +3 more
The messenger RNA for the lipoprotein of the E. coli outer membrane was found to code for a putative precursor, prolipoprotein, which has 20 additional amino acid residues extending from the amino terminus of the lipiprotein, and the complete amino acid sequence of the amino-terminal precursor region was determined to be as follows.
Journal of BacteriologyChromosomal Aberrations Associated with Mutations to Bacteriophage Resistance in <i>Escherichia coli</i>
248 Citations1965Roy Curtiss
The results with strains having /3,4,7,λ,pro−1,2 mutations were compatible with the hypothesis that this mutation caused a single break in the circular chromosome which prevented the normal association in the inheritance of the outside markers leu+ and lac+.
Proceedings of the National Academy of SciencesCell--cell interactions in conjugating Escherichia coli: role of traT protein in surface exclusion.
211 Citations1977Mark Achtman, Nicholas A. Kennedy +1 more
Two F cistrons, traS and traT, are independently responsible for part of the whole mechanism, which results in a greatly reduced ability to form stable mating aggregates, and thus also leads to reduced DNA transfer within the cell population.
Proceedings of the National Academy of SciencesDetection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.
209 Citations1978Chung Nan Chang, Günter Blobel +1 more
An inverted membrane vesicle fraction isolated from uninfected Escherichia coli and largely derived from the inner membrane has been shown to contain an endoproteolytic activity that cleaves nascent bacteriophage f1 pre-coat protein into two identifiable products.
Journal of VirologyAdsorption of Bacteriophages to Adhesions Between Wall and Membrane of <i>Escherichia coli</i>
171 Citations1968M. E. Bayer
Comparisons of the number of adsorbed phage particles observed in ultrathin sections and the expected number of phages per cell were in agreement, suggesting a sharing of receptive areas by the various phages.
Proceedings of the National Academy of SciencesSynthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.
170 Citations1977Hiroshi Inouye, Jon Beckwith
The presumed precursor can dimerize to form active enzyme without being processed, and the resultant enzyme appears to be more hydrophobic than the mature enzyme.
Proceedings of the National Academy of SciencesPartial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.
160 Citations1978R P Ambler, G. Kenneth Scott
Direct studies of the amino acid sequence of an Escherichia coli plasmid-coded penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) are in complete agreement with results derived from the translation of the DNA sequence of a related plasmids, apart from a single amino acid substitution.
NatureConjugation proteins encoded by the F sex factor
153 Citations1977Nicholas A. Kennedy, Lothar Beutin +4 more
Chimaeric plasmids carrying Eco RI fragments of the F sex factor have been used to identify proteins involved in conjugation and to assign them to tra cistrons.
Proceedings of the National Academy of SciencesAn Escherichia coli mutant with an amino acid alteration within the signal sequence of outer membrane prolipoprotein.
131 Citations1978Jing‐Jer Lin, H. Kanazawa +2 more
The data suggest, however, that proteolytic cleavage of prolipoprotein to form mature lipoprotein is not essential for the translocation and assembly of lipop protein into the outer membrane.
Journal of Molecular BiologyThe transcriptional control of fertility in F-like plasmids
129 Citations1977Neil Willetts
DNA from these phages was used in filter hybridization assays which indicated that during FinOP transfer inhibition of Flac or R100 the transcription of traJ is repressed, and that in the absence of the traJ gene product, the transfer operon is not transcribed.
Proceedings of the National Academy of SciencesPrecursors of three exported proteins in Escherichia coli.
124 Citations1978Linda L. Randall, S. J. S. Hardy +1 more
It is concluded that the larger forms of the exported proteins are precursors containing an extra sequence, when the intracellular protein elongation factor Tu is synthesized in vitro on free polysomes, it is not detectably larger than the authentic form.
Proceedings of the National Academy of SciencesMolecular cloning of DNA from F sex factor of Escherichia coli K-12.
106 Citations1976Ronald A. Skurray, Haruko Nagaishi +1 more
The molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F demonstrate one or more of the following F-specified functions: inhibition of female-specific phage (T7) multiplication, formation of F pili, surface exclusion, or immunity to lethal zygosis.
Proceedings of the National Academy of SciencesMutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein.
106 Citations1978Scott D. Emr, Martin A. Schwartz +1 more
Two mutant strains of Escherichia coli have been isolated in which the cellular location of an outer membrane protein, the phage lambda receptor, is altered, and it is suggested that these two mutations are in the portion of the lamB gene coding for a signal sequence and thereby block export of the protein.
Journal of BacteriologyMating aggregates in Escherichia coli conjugation
97 Citations1975Mark Achtman
A comparison of light microscopy, sucrose gradient centrifugation, and analysis with a modified Coulter counter indicated that the number of cells in mating aggregates is best equantitated using amodified Coulter counter.
Molecular and General Genetics MGGThe control region of the F sex factor DNA transfer cistrons: Restriction mapping and DNA cloning
94 Citations1978Russell Thompson, Mark Achtman
A restriction endonuclease map of EcoRI fragment f6 of F sex factor DNA was constructed and aligned with pre-existing physical and genetic maps to map the locations of the origin of DNA transfer and many of the transfer genes known to lie on f6.
Journal of BacteriologyAssignment of tra cistrons to EcoRI fragments of F sex factor DNA
94 Citations1978Mark Achtman, Ronald A. Skurray +5 more
The cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector is described, and it is found that traI is at least partially independent of transcription of the traA to traD operon.
Biochemical and Biophysical Research CommunicationsPrecursors of major outer membrane proteins of Escherichia coli
71 Citations1977Jun Sekizawa, Sumiko Inouye +2 more
Results strongly suggest that both the matrix protein and the to1G protein are produced from their precursors, the promatrixprotein and the protol-G protein, as is the case with the lipoprotein.
Journal of BacteriologyPolypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tn1 and Tn3
70 Citations1979Gordon Dougan, Michael W. Saul +3 more
Escherichia coli K-12 minicells were employed to examine polypeptides encoded by plasmids carrying wild-type and mutant Tn1 or Tn3 transposition elements, finding three related immunologically to beta-lactamase, the enzyme responsible for ampicillin hydrolysis.
Proceedings of the National Academy of SciencesVery stable prokaryotic messenger RNA in chromosomeless Escherichia coli minicells.
57 Citations1975S B Levy
The findings indicate that minicells contain normal degradative mechanisms for mRNA and point out the existence of a unique class of very stable cellular mRNA, and suggest that a class of cellular proteins is synthesized in bacteria without concomitant transcription and in the absence of association with chromosomal DNA.
Molecular and General Genetics MGGThe control region of the F sex factor DNA transfer cistrons: Physical mapping by deletion analysis
56 Citations1979Russell Thompson, Mark Achtman
A technique has been developed which allows the isolation of random deletions extending from unique restriction enzyme sites in plasmid DNA molecules and is used to map DNA transfer genes in the tra control region of F sex factor DNA.
Journal of Biological ChemistryA new form of structural lipoprotein of outer membrane of Escherichia coli.
40 Citations1977Simon Halegoua, Jun Sekizawa +1 more
It seems possible that this new form of thelipoprotine is a precursor of the lipoprotein (prolipoprotein) in the process of biosynthesis and assembly of the Lipoprotein in the outer membrane.
European Journal of BiochemistryCell Envelope Composition of <i>Escherichia coli</i> K12: A Comparison of the Cell Poles and the Lateral Wall
36 Citations1974E W Goodell, Uli Schwarz +1 more
The inner (cytoplasmic) membrane, outer membrane, and sacculus were prepared both from minicells and from filaments purified from a single culture.
Molecular and General Genetics MGGtra cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5
35 Citations1978Mark Achtman, Barica Kušećek +1 more
Hybrid plasmids obtained by cloning individual EcoRI and HindIII fragments of the conjugative plasmid, R6-5, were analyzed for their ability to complement transfer-deficient point mutations of Flac and found that the hybrid plasmide carrying oriT is mobilized by the F factor as well as by R 6-5.