Convergently functional, Rho-independent terminator in Salmonella typhimurium
Journal of BacteriologyPublished 1 July 1985Open access
M. Stella Carlomagno, Andrea Riccio, Carmelo B. Bruni
Citations45
SJR quartileQ2
SJR score1.09
SNIP0.85
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A typical Rho-independent terminator of transcription was found at the end of the histidine operon of Salmonella typhimurium, and termination of transcription at this site was very efficient, both in vivo and in vitro, and in both orientations.
Abstract
A typical Rho-independent terminator of transcription was found at the end of the histidine operon of Salmonella typhimurium. This site is used to terminate, in addition to the his operon mRNA, a 1,200-nucleotide RNA of unknown function transcribed on the opposite strand. The efficiency of termination of transcription at this site was investigated in vivo and in vitro by cloning of the terminator structure in either orientation in vector systems used to study regulatory signals. Termination of transcription at this site was very efficient, both in vivo and in vitro, and in both orientations.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
Molecular Cloning: A Laboratory Manual 2
129,982 Citations1989Joseph Sambrook, Elisabeth Fritsch +1 more
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
Methods in enzymology on CD-ROM/Methods in enzymology[57] Sequencing end-labeled DNA with base-specific chemical cleavages
14,691 Citations1980Allan M. Maxam, Walter Gilbert
Journal of Molecular BiologyLabeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
11,664 Citations1977Peter Rigby, Marianne Dieckmann +2 more
Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Proceedings of the National Academy of SciencesHybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
8,386 Citations1980Patricia S. Thomas
A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Annual Review of GeneticsREGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTION
2,724 Citations1979Martin Rosenberg, Donald Court
Nature New BiologyImproved Estimation of Secondary Structure in Ribonucleic Acids
2,287 Citations1973Ignacio Tinoco, Philip N. Borer +5 more
This method can be used for predicting and assessing possible secondary structures for recently determined RNA sequences and new experimental and theoretical results allow us to improve the method, without making it more complicated.
Methods in enzymology on CD-ROM/Methods in enzymology[68] Transcription maps of polyoma virus-specific RNA: Analysis by two-dimensional nuclease S1 gel mapping
1,391 Citations1980Jennifer M. Favaloro, Richard Treisman +1 more
This chapter describes the analysis of transcription maps of polyoma virus-specific RNA by 2-D nuclease S1 gel mapping and the standard procedures used for the preparation of viral nucleic acids and hybridization probes.
Journal of BacteriologyNature of Col E <sub>1</sub> Plasmid Replication in <i>Escherichia coli</i> in the Presence of Chloramphenicol
1,192 Citations1972Don B. Clewell
The colicinogenic factor E(1) (Col E( 1)) in Escherichia coli continues to replicate by a semiconservative mechanism in the presence of chloramphenicol for 10 to 15 hr, long after chromosomal deoxyribonucleic acid (DNA) synthesis has terminated.
NatureAttenuation in the control of expression of bacterial operons
758 Citations1981Charles Yanofsky
Bacterial operons concerned with the biosynthesis of amino acids are often controlled by a process of attenuation, where the amino acid in short supply means translation is stalled at the relevant codons of the transcript long enough for the succeeding segment of the transcripts to form secondary structures that allow the transcribing RNA polymerase molecule to proceed through a site that otherwise dictates termination of transcription.
Nucleic Acids ResearchRates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide
716 Citations1977James W. Casey, Norman Davidson
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide.
Annual Review of BiochemistryControl of Transcription Termination
622 Citations1978Sankar Adhya, Max E. Gottesman
The author revealed that RNA polymerase mutations located at the Site of Termination Site are linked to RHO mutations, and that pN acts at Many Sites and recognition Specificity of pN is recognized.
Proceedings of the National Academy of SciencesIsolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
569 Citations1980David A. Goldberg
The alcohol dehydrogenase (ADH) gene (Adh) of Drosophila melanogaster was isolated by utilizing a mutant strain in which the Adh locus is deleted, and two intervening sequences were identified within Adh by S1 nuclease mapping experiments.
Proceedings of the National Academy of SciencesOrganization of transcriptional signals in plasmids pBR322 and pACYC184.
365 Citations1981Dietrich Stüber, Hermann Bujard
Electron microscopic analysis of in vitro transcriptional complexes of pBR322 and pACYC184 revealed five and six major transcriptional units, respectively, in these two plasmid vectors, which permit a more predictable utilization of these cloning vehicles and also allow the reinterpretation of earlier cloning results.
Nucleic Acids ResearchThe nudeotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E. coil K12
260 Citations1981Zachary F. Burton, Richard R. Burgess +4 more
The resulting amino acid sequence agrees with previous information obtained about sigma including the amino acid composition, partial sequence data for the N-terminus, the highly acidic nature of the polypeptide, and the cleavage pattern at cysteines.
CellTermination of transcription and its regulation in the tryptophan operon of E. coli
250 Citations1981Terry Platt
Recent developments bearing on termination of transcription in E. coli, in the context of regulation of tryptophan operon mRNA synthesis, begin to answer several important questions about termination, including: What features control termination in the simplest systems in vitro?
CellThe operen that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12
245 Citations1983Zachary F. Burton, Carol A. Gross +2 more
The sigma subunit of E. coli RNA polymerase is encoded by the rpoD gene, and the structural genes rpsU and dnaG, which encode the 30S ribosomal protein S21 and DNA primase, respectively are identified.
Annual Review of GeneticsATTENUATION IN AMINO ACID BIOSYNTHETIC OPERONS
222 Citations1982Roberto Kolter, Charles Yanofsky
This paper presents a meta-anatomical model of the structure of the leader Peptide molecule and discusses its role in the development of inhibitory signalling mechanisms.
Nucleic Acids ResearchPrimary structure of the<i>ompF</i>gene that codes for a major outer membrane protein of<i>Escherichia coli</i>K-12
186 Citations1982Kaoru Inokuchi, Norihiro Mutoh +2 more
The codon usage in the ompF gene was highly nonrandom; the codons preferably utilized are those recognized by the most abundant species of isoaccepting tRNAs or those, among synonymous codons recognizing by the same tRNA, that can interact more properly with the anticodon.
Journal of Molecular BiologyRemoval of a terminator structure by RNA processing regulates int gene expression
141 Citations1984Ursula Schmeissner, Keith McKenney +2 more
NatureRegulation of the expression of the histidine operon in Salmonella typhimurium
138 Citations1974Takashi Kasai
A positive factor enhancing the expression of this operon binds at the promoter, but exerts its effect downstream on a DNA region which acts as an attenuator of transcription.
Journal of Molecular BiologyGene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction
111 Citations1980N. Rao Movva, Kenzo Nakamura +1 more
The DNA sequence of the ompA protein was found to consist of 325 amino acid residues and is produced from a secretory precursor, pro-ompA protein, having a signal peptide of 21 extra amino Acid residues at the amino terminal end, suggesting that the oMPA protein can be incorporated without 30% of the protein from its carboxyl terminus into the outer membrane.
Journal of Biological ChemistryMessenger ribonucleic acid of the lipoprotein of the Escherichia coli outer membrane. II. The complete nucleotide sequence.
100 Citations1980K. Nakamura, Robert M. Pirtle +3 more
The complete nucleotide sequence of the mRNA for the outer membrane lipoprotein from Escherichia coli has been determined and has several unique features, including many repeating sequences including one repeat of 40 nucleotides.
ScienceStudying Promoters and Terminators by Gene Fusion
73 Citations1983Martin Rosenberg, Ana B. Chepelinsky +1 more
Prokaryotic gene control signals can be isolated, compared, and characterized by precise fusion in vitro to the Escherichia coli galactokinase gene (galK), which provides both a simple assay and genetic selection.
Nucleic Acids ResearchThe distal end of the ribosomal RNA operon rrnD of Eschenchia coli contains a tRNA<sub>1</sub><sup>thr</sup>gene, two 5S rRNA genes and a transcription terminator
60 Citations1980Gregg Duester, W. Michael Holmes
The distal region of the Escherichia coli ribosomal RNA operon rrnD has been sequenced to identify transfer RNA genes which might be contained within this transcriptional unit, and to define a possible transcription termination site.
Journal of BacteriologyStructure and function of the internal promoter (hisBp) of the Escherichia coli K-12 histidine operon
43 Citations1983V Grisolia, Andrea Riccio +1 more
The entire histidine operon of Escherichia coli K-12 was cloned in the vector plasmid pBR313, and a complete restriction map of the operon was determined, finding the sequence of a fragment of DNA 665 base pairs long, comprising the distal portion of the hisC gene, the proximal portion of hisB gene, and the internal transcription initiation site hisBp.
Journal of BacteriologyCloning and expression of the distal portion of the histidine operon of Escherichia coli K-12
41 Citations1982V Grisolia, M. Stella Carlomagno +1 more
The operator-distal genes hisBHAFI(E) of the Escherichia coli K-12 histidine operon were mapped on a DNA fragment 4,500 base pairs long, and the cloned genes were expressed in appropriate hosts, independent of the orientation of the DNA fragment.
Current topics in cellular regulationRegulation of the Histidine Operon: Translation-Controlled Transcription Termination (A Mechanism Common to Several Biosynthetic Operons)
39 Citations1981Francesco Blasi, Carmelo B. Bruni
This chapter discusses the regulation of the histidine operon, which represents a new kind of regulatory mechanism, well differentiated from that of the repressor-operator interaction, that utilizes the protein synthesis machinery thus, linking theregulation of one single system to the growth of the cell.
Nucleic Acids ResearchIdentification, nucleotide sequence and expression of the regulatory region of the histidine operon of Escherichia coli K-12
39 Citations1981Pasquale Verde, Rodolfo Frunzio +3 more
Another promoter was identified on the same fragment of deoxyribonucleic acid by in vitro transcription, DNA sequencing and RNA polymerase binding and both promoters are very A-T rich and are separated by a G-C rich region containing a palyndromic structure.
Proceedings of the National Academy of SciencesIn vivo and in vitro detection of the leader RNA of the histidine operon of Escherichia coli K-12.
32 Citations1981Rodolfo Frunzio, Carmelo B. Bruni +1 more
The existence of the Histidine leader RNA lends support to the regulatory mechanism which postulates that regulation of the histidine operon is dependent on the alternative secondary structures that the leader RNA may assume, depending on whether or not the histazine-rich leader peptide is translated.
Methods in enzymology on CD-ROM/Methods in enzymology[8] Repair of overlapping DNA termini
30 Citations1980Howard M. Goodman
Journal of BacteriologyInternal promoters of the his operon in Salmonella typhimurium
24 Citations1983Molly B. Schmid, J R Roth
Two internal promoters in the his operon of Salmonella typhimurium have been precisely mapped genetically and it is argued that they are not likely to be fortuitous message start sites, but may play a physiologically important role in operon expression.
Molecular and General Genetics MGGGene organization in the distal part of the Salmonella typhimurium histidine operon and determination and sequence of the operon transcription terminator
17 Citations1983Maria Stella Carlomagno, Francesco Blasi +1 more
Several transducing phages, carrying different deletions of the Salmonella thyphimurium histidine operon were constructed and mapped to obtain fragments of DNA comprising different regions of the operon which were subcloned in plasmid vectors.