Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (<i>APH</i>) of <i>Bacillus circulans</i>. Self-defence mechanism in antibiotic-producing organisms
Biochemical JournalPublished 15 January 1986Open access
C.J. Herbert, Mohammed G. Sarwar, Sarbjit S. Ner, Ian G. Giles, Muhammad Akhtar
Citations22
SJR quartileQ1
SJR score2.06
SNIP0.98
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TL;DR
The APH gene of a butirosin-producing Bacillus circulans was cloned and shown to be expressed in Escherichia coli and Streptomyces lividans, indicating that the genes may have a common origin.
Abstract
The APH gene of a butirosin-producing Bacillus circulans was cloned and shown to be expressed in Escherichia coli and Streptomyces lividans. The gene was sequenced and a possible developmentally regulated promoter identified. When the deduced protein sequence was compared with those from transposon Tn5, transposon Tn903, Streptomyces fradiae, Staphylococcus aureus and Streptococcus faecalis, significant homology was found, indicating that the genes may have a common origin.
Keywords
Biochemistry, Genetics and Molecular Biology
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