Increase in intranuclear nuclear factor κB and decrease in inhibitor κB in mononuclear cells after a mixed meal: evidence for a proinflammatory effect
American Journal of Clinical NutritionPublished 1 April 2004Open access
Ahmad Aljada, Priya Mohanty, Husam Ghanim, Toufic Abdo, Devjit Tripathy, Ajay Chaudhuri
Citations239
SJR quartileQ1
SJR score2.11
SNIP1.75
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Data show that the intake of a mixed meal results in significant inflammatory changes characterized by a decrease in IkappaBalpha and an increase in NF-kappaB binding, plasma CRP, and the expression of IKKalpha, IKKbeta, and p47(phox) subunit.
Abstract
These data show that the intake of a mixed meal results in significant inflammatory changes characterized by a decrease in IkappaBalpha and an increase in NF-kappaB binding, plasma CRP, and the expression of IKKalpha, IKKbeta, and p47(phox) subunit. These proinflammatory changes are probably relevant to the state of chronic hypertension and obesity and to its association with atherosclerosis.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
New England Journal of MedicineAtherosclerosis — An Inflammatory Disease
21,594 Citations1999Russell Ross
Annual Review of ImmunologyFunction and Activation of NF-kappaB in the Immune System
4,698 Citations1994Patrick A. Baeuerle, Thomas Henkel
The inhibition of NF-kappa B activation by antioxidants and specific protease inhibitors may provide a pharmacological basis for interfering with these acute processes in suppressing toxic/septic shock, graft-vs-host reactions, acute inflammatory reactions, severe phase response, and radiation damage.
New England Journal of MedicineNuclear Factor-κB — A Pivotal Transcription Factor in Chronic Inflammatory Diseases
4,671 Citations1997Peter J. Barnes, Michael Karin
In chronic inflammatory diseases, such as asthma, rheumatoid arthritis, inflammatory bowel disease, and psoriasis, several cytokines recruit activated immune and inflammatory cells to the site of lesions, thereby amplifying and perpetuating the inflammatory state.
JAMAC-Reactive Protein, Interleukin 6, and Risk of Developing Type 2 Diabetes Mellitus
4,431 Citations2001Aruna D. Pradhan
Elevated levels of CRP and IL-6 predict the development of type 2 DM, and data support a possible role for inflammation in diabetogenesis.
Arteriosclerosis Thrombosis and Vascular BiologyC-Reactive Protein in Healthy Subjects: Associations With Obesity, Insulin Resistance, and Endothelial Dysfunction
2,463 Citations1999John Yudkin, Coen D.A. Stehouwer +2 more
The data suggest that adipose tissue is an important determinant of a low level, chronic inflammatory state as reflected by levels of interleukin-6, tumor necrosis factor-alpha, and C-reactive protein, and that infection with H pylori, C pneumoniae, and cytomegalovirus is not.
Nucleic Acids ResearchA rapid micrqpreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells
2,338 Citations1991Nancy C. Andrews, Douglas V. Faller
The aim in developing this micropreparation procedure was to easily and rapidly extract DNA-binding proteins from small numbers of cells, and it gives an excellent yield, comparable to that of the large scale Dignam protocol with minimal proteolysis.
JAMAIntensive Lifestyle Changes for Reversal of Coronary Heart Disease
1,710 Citations1998Dean Ornish
More regression of coronary atherosclerosis occurred after 5 years than after 1 year in the experimental group, and in the control group, coronary Atherosclerosis continued to progress and more than twice as many cardiac events occurred.
The American Journal of CardiologyEffect of a Single High-Fat Meal on Endothelial Function in Healthy Subjects
796 Citations1997Robert A. Vogel, Mary C. Corretti +1 more
It is demonstrated that a single high-fat meal transiently impairs endothelial function and identifies a potential process by which a high-Fat diet may be atherogenic independent of induced changes in cholesterol.
The Journal of Clinical Endocrinology & MetabolismInsulin Inhibits Intranuclear Nuclear Factor κB and Stimulates IκB in Mononuclear Cells in Obese Subjects: Evidence for an Anti-inflammatory Effect?
667 Citations2001Paresh Dandona, Ahmad Aljada +5 more
It is concluded that insulin has a potent acute anti-inflammatory effect including a reduction in intranuclear NFkappaB, an increase in Ikappa B, and decreases in ROS generation, p47(phox) subunit, plasma soluble intercellular adhesion molecule-1, monocyte chemoattractant protein-1 (MCP-1), and plasminogen activator inhibitor- 1 (PAI-1).
The Journal of Clinical Endocrinology & MetabolismGlucose Challenge Stimulates Reactive Oxygen Species (ROS) Generation by Leucocytes
599 Citations2000Priya Mohanty, Wael Hamouda +4 more
It is concluded that glucose intake stimulates ROS generation and p417phox of NADPH oxidase; increases oxidative load and causes a fall in alpha-tocopherol concentration.
The Journal of Clinical Endocrinology & MetabolismTumor Necrosis Factor-α in Sera of Obese Patients: Fall with Weight Loss
537 Citations1998Paresh Dandona, Ruth S. Weinstock +4 more
This study investigated whether serum TNFα concentrations are elevated in obese subjects, whether they fall after weight loss, and whether this fall parallels the fall in insulin release after glucose challenge.
Journal of Clinical Investigationp47phox is required for atherosclerotic lesion progression in ApoE–/– mice
481 Citations2001Patricia A. Barry-Lane, Cam Patterson +5 more
These studies provide convincing support for the hypothesis that superoxide generation in general, and NADPH oxidase in particular, have a requisite role in atherosclerotic lesion formation, and they provide a rationale for further studies to dissect the contributions of ROS to vascular lesions formation.
JAMAEffect of Antioxidant Vitamins on the Transient Impairment of Endothelium—Dependent Brachial Artery Vasoactivity Following a Single High-Fat Meal
455 Citations1997Gary D. Plotnick
A single high-fat meal transiently reduces endothelial function for up to 4 hours in healthy, normocholesterolemic subjects, probably through the accumulation of triglyceride-rich lipoproteins, suggesting an oxidative mechanism.
The Journal of Clinical Endocrinology & MetabolismThe Suppressive Effect of Dietary Restriction and Weight Loss in the Obese on the Generation of Reactive Oxygen Species by Leukocytes, Lipid Peroxidation, and Protein Carbonylation1
372 Citations2001Paresh Dandona, Priya Mohanty +7 more
American Journal of Clinical NutritionBoth lipid and protein intakes stimulate increased generation of reactive oxygen species by polymorphonuclear leukocytes and mononuclear cells
240 Citations2002Priya Mohanty, Husam Ghanim +4 more
Both fat and protein intakes stimulate ROS generation in MNCs and PMNs and the increase in ROS generation lasted 3 h after cream intake and 1 h after protein intake, which caused a significant and prolonged increase in lipid peroxidation.
The Journal of Clinical Endocrinology & MetabolismSuppression of Nuclear Factor-κB and Stimulation of Inhibitor κB by Troglitazone: Evidence for an Anti-inflammatory Effect and a Potential Antiatherosclerotic Effect in the Obese
219 Citations2001Husam Ghanim, Rajesh Garg +6 more
It is concluded that troglitazone has an antiinflammatory action that may contribute to its putative antiatherosclerotic effects.
Biochemical JournalThe inhibition by diphenyleneiodonium and its analogues of superoxide generation by macrophages
209 Citations1987John T. Hancock, Owen Jones
Its sensitivity to DPI suggests that selective prevention of radical generation by macrophages in vivo is possible, and the O2(.-)-generating oxid enzyme appears to have components in common with the NADPH oxidase of neutrophils, despite differences in activity.
MetabolismMeal-induced oxidative stress and low-density lipoprotein oxidation in diabetes: The possible role of hyperglycemia
198 Citations1999Antonio Ceriello, Nadia Bortolotti +8 more
It is suggested that postprandial hyperglycemia may contribute to oxidative stress in diabetic patients, providing a mechanistic link between hyper glycemia and diabetic vascular disease.
The Journal of Clinical Endocrinology & MetabolismInsulin Inhibits NFκB and MCP-1 Expression in Human Aortic Endothelial Cells
186 Citations2001Ahmad Aljada, Husam Ghanim +2 more
It is concluded that insulin at physiologically relevant concentrations exerts an inhibitory effect on the cardinal pro-inflammatory transcription factor NFkappaB and the pro- inflammatory chemokine MCP-1; these effects suggest an anti-inflammatory and potential anti-atherogenic effects of insulin.
The Journal of Clinical Endocrinology & MetabolismInsulin Inhibits the Expression of Intercellular Adhesion Molecule-1 by Human Aortic Endothelial Cells through Stimulation of Nitric Oxide1
167 Citations2000Ahmad Aljada, Rana Saadeh +3 more
It is concluded that insulin reduces the expression of the proinflammatory adhesion molecule ICAM-1 through an increase in theexpression of NOS and NO generation and that insulin may have a potential antiinflammatory and antiatherosclerotic effect rather than a proatherosclerosis effect.
Trends in Endocrinology and MetabolismRAGE: A New Target for the Prevention and Treatment of the Vascular and Inflammatory Complications of Diabetes
163 Citations2000Ann Marie Schmidt, David M. Stern
The studies provide mechanistic insights into complications within the macrovasculature and those ensuing from an exaggerated host response to invading bacteria, and suggest that blockade of RAGE might provide a potent and safe strategy for the prevention of complications that typify long-term diabetes.
CirculationCarvedilol Inhibits Reactive Oxygen Species Generation by Leukocytes and Oxidative Damage to Amino Acids
141 Citations2000Paresh Dandona, Rajaram Karne +4 more
It is concluded that carvedilol significantly inhibits ROS generation by leukocytes and oxidative conversion of phenylalanine to m- and o-tyrosine and antioxidant effect in humans in vivo.
The Journal of Clinical Endocrinology & MetabolismRAPID COMMUNICATION: Inhibitory Effect of a Two Day Fast on Reactive Oxygen Species (ROS) Generation by Leucocytes and Plasma Ortho-Tyrosine and Meta-Tyrosine Concentrations
134 Citations2001P Dandona, Priya Mohanty +5 more
A 48h fast may reduce ROS generation, total oxidative load and oxidative damage to amino acids in polymorphonuclear leucocytes and mononuclear cells.
Seminars in Thrombosis and HemostasisRAGE: A Multiligand Receptor Contributing to the Cellular Response in Diabetic Vasculopathy and Inflammation
130 Citations2000Ann-Marie Schmidt, M. Hofmann +3 more
Data suggest that RAGE has the potential to function as a progression factor in a range of disorders in which its ligands accumulate and the chronic juxtaposition of ligand and receptor triggers sustained cellular perturbation favoring mechanisms eventuating in tissue injury rather than those that would restore homeostasis.
Journal of the American College of CardiologyEnhanced peripheral vasodilation in humans after a fatty meal
122 Citations2000Olli T. Raitakari, Nicole Chit Hang Lai +4 more
It is concluded that eating a fatty meal induces vasodilation and increases resting and stimulated FBF and that these observations are probably mediated by postprandial changes in insulin and/or triglyceride levels.
PubMedNF-kappa B is activated during acute inflammation in vivo in association with elevated endothelial cell adhesion molecule gene expression and leukocyte recruitment.
107 Citations1995Anthony M. Manning, Frank P. Bell +7 more
NF-kappa B activation is therefore an early event in the initiation of acute inflammation in vivo and this molecular pathway may be of consequence in the pathogenesis of acute inflammatory disease.
Cellular ImmunologyEndotoxin tolerance: In vivo regulation of tumor necrosis factor and interleukin-1 synthesis is at the transcriptional level
86 Citations1992S Zuckerman, Glenn F. Evans
The hyporesponsive state associated with endotoxin tolerance is characterized by transcriptional regulation of both TNF and IL-1 synthesis, which is consistent with a decrease in transcriptional activity by nuclear run-on assays for both cytokines and a reduced amount of the nuclear-associated transcription factor NF-KB.
Annals of the New York Academy of SciencesAtherosclerosis as an Inflammatory Process
60 Citations1985Colin J. Schwartz, Anthony J. Valente +4 more
In this brief review, the roles of the monocyte-macrophage in atherogenesis, with emphasis on the recruitment to the arterial wall is addressed, and summary data on the SMC-derived chemoattractant protein and also on the temporal evolution of monocytes into cholesteryl ester-rich foam cells are presented.
American Journal of Clinical PathologyA Rapid, Specific assay for Superoxide Release from Phagocytes in Small Volumes of Whole Blood
57 Citations1992Michael F. Tosi, Akbar Hamedani
A rapid, specific assay was developed to measure the release of superoxide anion from stimulated phagocytic cells in small volumes of whole blood, based on the chemiluminescence that results when lucigenin (bis-N-methyl acridinium nitrate) is reduced by superoxide.
MetabolismEffect of hydrocortisone on oxygen free radical generation by mononuclear cells
54 Citations1998Paresh Dandona, Kuldip Thusu +3 more
Serum cortisol concentrations were markedly elevated over basal and remained elevated throughout the first 8 hours of the experiment, returning to baseline at 24 hours, suggesting that inhibition of ROS generation by HC (and other glucocorticoids) may have a role to play in mediating the antiinflammatory action of corticosteroids.
The Journal of Clinical Endocrinology & MetabolismIncreased IκB Expression and Diminished Nuclear NF-κB in Human Mononuclear Cells following Hydrocortisone Injection
50 Citations1999Ahmad Aljada, Husam Ghanim +5 more
It is demonstrated that hydrocortisone induces IkappaB and suppresses NF-kappaB expression in MNC in parallel, which further reduces the translocation of NF- kappaB into the nucleus thus preventing the expression of proinflammatory genes.
MetabolismAcute suppressive effect of hydrocortisone on p47phox subunit of nicotinamide adenine dinucleotide phosphate oxidase
24 Citations2001P Dandona, Ahmad Aljada +4 more
It would appear that the decrease in intranuclear NFkappaB and an increase in IL-10 may cause the inhibitory modulation on p47(phox) subunit and ROS generation by MNC following hydrocortisone and other glucocorticoids.
NatureA regulator branches out
22 Citations2003Alain Israël
The cellular signalling pathway that leads to activation of the NF-κB protein has been studied for many years, and one might think that there's little left to learn, but it still has some surprises in store.