Sequence of a Probable Potassium Channel Component Encoded at <i>Shaker</i> Locus of <i>Drosophila</i>
SciencePublished 14 August 1987
Bruce L. Tempel, Diane M. Papazian, Thomas L. Schwarz, Yuh Nung Jan, Lily Yeh Jan
Citations650
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
The sequence of two complementary DNA clones from the Shaker locus of Drosophila supports the hypothesis that Shaker encodes a structural component of a voltage-dependent K+ channel and suggests a conserved mechanism for voltage activation.
Abstract
Potassium currents are crucial for the repolarization of electrically excitable membranes, a role that makes potassium channels a target for physiological modifications that alter synaptic efficacy. The Shaker locus of Drosophila is thought to encode a K+ channel. The sequence of two complementary DNA clones from the Shaker locus is reported here. The sequence predicts an integral membrane protein of 70,200 daltons containing seven potential membrane-spanning sequences. In addition, the predicted protein is homologous to the vertebrate sodium channel in a region previously proposed to be involved in the voltage-dependent activation of the Na+ channel. These results support the hypothesis that Shaker encodes a structural component of a voltage-dependent K+ channel and suggest a conserved mechanism for voltage activation.
Keywords
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