Selective translation initiation on bicistronic simian virus 40 late mRNA
Journal of VirologyPublished 1 July 1987Open access
David Grass, James L. Manley
Citations16
SJR quartileQ1
SJR score1.28
SNIP0.90
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TL;DR
A simian virus 40 (SV40) mutant, pSVAdL, efficiently synthesizes agnoprotein, a protein encoded by the leader region of the same mRNA that encodes VP1, which supports a scanning model of translation initiation and suggests that internal translational reinitiation does not occur efficiently in this situation.
Abstract
We described previously a simian virus 40 (SV40) mutant, pSVAdL, that was defective in synthesis of the late viral protein VP1. This mutant, which contains a 100-base-pair fragment of adenovirus DNA encompassing the major late promoter inserted in the SV40 late promoter region (SV40 nucleotide 294), efficiently synthesizes agnoprotein, a protein encoded by the leader region of the same mRNA that encodes VP1. When the agnoprotein AUG initiation codon in pSVAdL was mutated to UUG, agnoprotein synthesis was abolished, and VP1 synthesis was elevated to wild-type levels. Because levels of late mRNA synthesis were not affected by this mutation, these results support a scanning model of translation initiation and suggest that internal translational reinitiation does not occur efficiently in this situation.
Keywords
Biochemistry, Genetics and Molecular Biology
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