Localization of Inhibition of replicon initiation to damaged regions of DNA
Journal of Molecular BiologyPublished 1 July 1977
Lawrence F. Povirk
Citations44
SJR quartileQ1
SJR score2.21
SNIP1.13
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TL;DR
Iradiation of synchronized S phase Chinese hamster ovary cells with 313 nm wavelength light inhibited the initiation of replicons in DNA substituted with bromodeoxyuridine but did not affect the replication of unsubstituted DNA occurring simultaneously in the same cells.
Abstract
Irradiation of synchronized S phase Chinese hamster ovary cells with 313 nm wavelength light inhibited the initiation of replicons in DNA substituted with bromodeoxyuridine but did not affect the replication of unsubstituted DNA occurring simultaneously in the same cells. This result suggests that initiation is inhibited only in the region of the chromosome that sustains damage. Calculations of the frequency of damaged sites suggest that this inhibition, and probably a similar effect induced by X-rays, could be mediated by conformational changes in regions of individual DNA molecules up to several hundred μm in length.
Keywords
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