A direct link between core histone acetylation and transcriptionally active chromatin.
The EMBO JournalPublished 1 May 1988Open access
Tim R. Hebbes, Alan W. Thorne, Colyn Crane‐Robinson
Citations926
SJR quartileQ1
SJR score4.82
SNIP1.93
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TL;DR
The results demonstrate directly that transcriptionally active genes carry acetylated core histones in chromatin fragments fractions from the nuclei of 15‐day chicken embryo erythrocytes.
Abstract
An antiserum raised against chemically acetylated histone H4 was found to recognize the epitope epsilon-N-acetyl lysine. Affinity-purified antibodies were used to fractionate oligo- and mononucleosomal chromatin fragments from the nuclei of 15-day chicken embryo erythrocytes. Antibody-bound chromatin was found to contain elevated levels of acetylated core histones. On probing with sequences of alpha D globin, an actively transcribed gene, the antibody-bound chromatin was 15- to 30-fold enriched relative to the input chromatin. Using ovalbumin sequences as a probe, no enrichment was observed. The results demonstrate directly that transcriptionally active genes carry acetylated core histones.
Keywords
Biochemistry, Genetics and Molecular Biology
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The EMBO JournalA direct link between core histone acetylation and transcriptionally active chromatin.
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