Top Research Papers on CRISPR

This Perspective presents a detailed summary of which micro/nanotechnologies play roles in the advancement of CRISPR diagnosis and how they are involved, and discusses upcoming opportunities and improvements and how micro-nanoteschnologies will continue to play key roles.

The CRISPR/Cas9-mediated lateral flow nucleic acid assay satisfies some of the characteristics of a next-generation molecular diagnostics tool due to its rapidity and accuracy, allowing for point-of-care use without the need for technical expertise and complex ancillary equipment.

Following the above attempts, the developed CRISPR/Cas12a biosensors not only present an ultrasensitive assay behavior toward these model non-nucleic acid analytes but also can serve as a formidable toolbox for determining real samples including single cell lysates and human plasma, proving a good practical application capacity.

A clustered regularly interspaced short palindromic repeats-mediated surface-enhanced Raman scattering (SERS) assay for multidrug-resistant (MDR) bacteria is reported, which may prove effective for the accurate diagnosis of MDR bacterial pathogens, thus preventing severe infection by ensuring appropriate antibiotic treatment.

A novel design for a colorimetric gene-sensing platform based on the CRISPR/Cas system shows superior characteristics, such as probe universality, compatibility with isothermal reaction conditions, on-site detection capability, and high sensitivity; thus represents a robust next-generation gene detection platform.

It is suggested that this ultrasensitive amplification-free detection system for nucleic acids can be widely applied to the precise and early diagnosis of viral infections, cancers, and several genetic diseases.

This work presents the first evidence of large palindromic structures in a plant sex chromosome, based on a highly contiguous assembly of the W chromosome of the dioecious shrub Salix purpurea, and highlights the intrinsic importance of these features in adaptive evolution in the absence of recombination.

This paper proposes an Efficient and Effective Incomplete Multi-view Clustering (EE-IMVC) algorithm, which proposes to impute each incomplete base matrix generated by incomplete views with a learned consensus clustering matrix to address issues of intensive computational and storage complexities, over-complicated optimization and limitedly improved clustering performance.

It is shown that LNP delivery of CRISPR-Cas9 enables repeated injections into skeletal muscle and leads to restored dystrophin expression in multiple muscle groups.

The fast locality-sensitive hashing–based clustering (FLSHclust) algorithm is developed, which performs deep clustering on massive datasets in linearithmic time, and uncovered more than 200 new functional systems linked to CRISPR, a technology editing DNA.

This work describes the proposed method as Structurally Regularized Deep Clustering (SRDC), where it enhances target discrimination with clustering of intermediate network features, and enhance structural regularization with soft selection of less divergent source examples.

ABSTRACT Current stellar evolution models predict a dearth of black holes (BHs) with masses $\gtrsim \! 50\, \rm M_\odot$ and $\lesssim \! 5\, \rm M_\odot$, and intermediate-mass black holes (IMBHs; $\sim \! 10^2\!-\! 10^5\rm\, M_\odot$) have not yet been detected beyond any reasonable doubt. A natural way to form massive BHs is through repeated mergers, detectable via gravitational wave emission with current LIGO/Virgo or future LISA and ET observations. Nuclear star clusters (NSCs) have masses and densities high enough to retain most of the merger products, which acquire a recoil kick at the...

A genetically encoded model in which L1 and B1/Alu repeats blueprint chromatin macrostructure is proposed, which explains the robustness of genome folding into a common conserved core, on which dynamic gene regulation is overlaid across cells.

A robust bi-stochastic graph regularized matrix factorization (RBSMF) framework for data clustering is proposed, which is more robust than the commonly used general loss function.

Abstract Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain largely unknown. Here, we report the discovery of new Acrs by assaying candidate genes adjacent to a conserved Acr-associated (Aca) gene, aca5 , against a panel of six type I systems: I–F ( Pseudomonas , Pectobacterium , and Serratia ), I–E ( Pseudomonas and Serratia ), and I–C ( Pseudomonas )...

It is found that STRs can modulate transcription factor (TF)-DNA affinities and on rates by up to 70-fold by directly binding TF DNA-binding domains, with energetic impacts approaching or exceeding mutations to consensus sites.

This review focuses on anti-CRISPRs (Acrs), proteins produced by viruses and other mobile genetic elements that can potently inhibit CRISPR-Cas systems.

Four ongoing research hotspots in molecular diagnostics-target preamplification-free detection, microRNA (miRNA) detection, non-nucleic-acid detection, and SARS-CoV-2 detection-are also covered.

The study provides insights into the genomic properties of structural variant classes and short tandem repeats that are associated with gene expression and human traits and finds properties, such as length of variation, that affect the association.

Abstract LIGO and Virgo have reported the detection of GW190521, from the merger of a binary black hole (BBH) with a total mass around 150 M ⊙ . While current stellar models limit the mass of any black hole (BH) remnant to about 40–50 M ⊙ , more massive BHs can be produced dynamically through repeated mergers in the core of a dense star cluster. The process is limited by the recoil kick (due to anisotropic emission of gravitational radiation) imparted to merger remnants, which can escape the parent cluster, thereby terminating growth. We study the role of the host cluster metallicity and escap...