Macrolide compounds, ivermectin and milbemycin D, stimulate chloride channels sensitive to gabaergic drugs in cultured chick spinal neurons
Comparative Biochemistry and Physiology Part C Comparative PharmacologyPublished 1 January 1989
Jun Yamazaki, Kinzo Matsumoto, Hideki Ono, Hideomi Fukuda
Citations17
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TL;DR
The results suggest that IVM and MMD directly activate the Cl- channel through their specific sites modulated by GABAergic drugs.
Abstract
1. The effects of ivermectin (IVM) and milbemycin D (MMD) on cultured embryonic spinal neurons were investigated, using the whole-cell recording technique. 2. IVM and MMD caused Cl- current under voltage-clamp conditions. 3. The reversal potential of the MMD-evoked Cl- current was shifted in a positive direction when half of the external Cl- was substituted by isethionate. 4. The MMD-evoked current was inhibited by picrotoxinin and bicuculline, whereas it was enhanced by diazepam. 5. Different characteristics of desensitization were observed between the MMD- (IVM-) and muscimol-induced effects. 6. These results suggest that IVM and MMD directly activate the Cl- channel through their specific sites modulated by GABAergic drugs.
Keywords
PsychologyNeuroscienceBiochemistry, Genetics and Molecular Biology
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